Transcriptomics,Genomics

Dataset Information

155

RNA-seq identified KLF16 as a negative regulator of adipogenesis


ABSTRACT: We performed high throughput RNA sequencing at preadipocyte (D0) and differentiated adipocyte (D7) of primary brown preadipocyte and found that Kruppel-like factor 16 (KLF11) gene that was downregulated in D7 was a novel negative regulator of adipogenesis. Overall design: To explore global view of gene expression during adipogenesis, RNA-seq was performed in the primary cultured brown preadipocyte (D0) and brown adipocyte after 7 day differentiation (D7). Compared with D0, 6,668 genes were identified as 2 fold differentially expressed genes (DEGs) at D7 including 2,836-upregulated genes and 3,832 down-regulated genes.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Lee Sunwoo  

PROVIDER: GSE73279 | GEO | 2016-09-21

SECONDARY ACCESSION(S): PRJNA296514

REPOSITORIES: GEO

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Publications

RNA-Seq Analysis Reveals a Negative Role of KLF16 in Adipogenesis.

Jang Min-Kyung MK   Lee Sunwoo S   Jung Myeong Ho MH  

PloS one 20160909 9


In this study, we performed high throughput RNA sequencing at the preadipocyte (D0) and differentiated adipocyte (D7) stages of primary brown preadipocyte differentiation in order to characterize the transcriptional events regulating differentiation and function. Compared to the preadipocyte stage (D0), 6,668 genes were identified as differentially expressed genes (DEGs) with a fold change of ≥ 2.0 at the differentiated adipocyte stage (D7). Several adipogenic genes including peroxisome prolifer  ...[more]

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