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Effect of Cnot7 expression on 4T1 mammary tumor cell line transcriptome

ABSTRACT: The goal of this study was to determine what the effect of modulating Cnot7 expression levels would have on the steady state transcription program of the 4T1 mammary tumor cell line.

ORGANISM(S): Mus musculus

PROVIDER: GSE73296 | GEO | 2015/09/22

SECONDARY ACCESSION(S): PRJNA296556

REPOSITORIES: GEO

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Effect of Cnot7 expression on 4T1 mammary tumor cell line transcriptome

Project description:The goal of this study was to determine what the effect of modulating Cnot7 expression levels would have on the steady state transcription program of the 4T1 mammary tumor cell line. Three biological replicates for over expression compared to vector control. Three biological replicates for two different shRNAs versus shScramble control

2015-09-22 | E-GEOD-73296 | biostudies-arrayexpress

Expression analysis of the effect of overexpression of Cebpb shRNA in mouse 4T1 mammary tumor cells

2012-09-28 | GSE40825 | GEO

Expression analysis of the effect of overexpression of Cebpb shRNA in mouse 4T1 mammary tumor cells

Project description:Investigation of whole genome gene expression level changes in mouse 4T1 mammary tumors expressing Cebpb shRNA, compared to 4T1 tumors expressing control shRNA. Analysis of mouse 4T1 mammary tumors expressing Cebpb shRNA compared to control shRNA are further described in Johansson & Berg et al 2012. A 10 chip study using total RNA recovered from five separate 4T1 tumors expressing Cebpb shRNA and five separate 4T1 tumors expressing control shRNA. All tumors were surgically removed after subcutaneous implantation in syngeneic BALB/c mice two weeks earlier. Each chip measures the expression level of 44,170 genes from Mus Musculus with fourteen 24-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.

2012-09-28 | E-GEOD-40825 | biostudies-arrayexpress

RNA IP Seq analysis of Cnot7 in 4T1 mammary tumor cells

Project description:Native RNA immunoprecipitation of Cnot7-bound transcripts. Abstract: Accumulating evidence supports the role of an aberrant transcriptome as a driver of tumor cell metastatic potential. Deadenylation is a general regulatory node for post-transcriptional control by microRNAs and other determinants of RNA stability. We show here that CCR4-NOT subunit CNOT7 is a deadenylase-dependent driver of tumor cell autonomous metastatic potential. Metastasis promotion by CNOT7 is dependent on contact with CNOT1 and TOB1. We further show TOB1 independently drives metastasis. RNA-immunoprecipitation and integrated transcriptome wide analyses reveal that CNOT7-regulated transcripts are enriched for a tripartite 3’UTR motif bound by RNA-binding proteins known to complex with CNOT7, TOB1, and CNOT1. Collectively, our data support a model of CNOT7, TOB1, CNOT1, and RNA-binding proteins collectively exerting post-transcriptional control on a metastasis suppressive transcriptional program to drive tumor cell metastasis. 48 total samples, 4-5 biological replicates, two forms of control: input samples and vector controls

2015-09-23 | E-GEOD-73366 | biostudies-arrayexpress

RNA IP Seq analysis of Cnot7 in 4T1 mammary tumor cells

2015-09-23 | GSE73366 | GEO

Single cell RNA-seq of 4T1-T vasculogenic mimicry-competent mammary tumors

Project description:We have previously described a sub-clones of the 4T1 mammary carcinoma cell line that are proficient for vasculogenic mimciry (VM), namely 4T1-E and 4T1-T. In vitro assays suggest that not all cells within these lines a VM-competent. To explore subsets of cells within tumors derived from these cells that may display VM properties we utilized single cell RNA-Seq of 4T1-T mammary fat-pad tumors.

2023-07-26 | GSE230642 | GEO

Transcript profiling of mouse 4T1 tumors grown in preirradiated vs. nonirradiated mammary tissue

Project description:The present study was designed to identify genes induced by irradiation in the 4T1 breast cancer model mimicking aggressive local relapse after radiotherapy. For this purpose, we obtained the transcriptomes of 4T1 tumors grown in either preirradiated (IRR+4T1) or non-irradiated (4T1) mammary tissue.

2017-08-31 | GSE64193 | GEO

Gene expression of clonal subpopulations derived from the 4T1 murine mammary carcinoma cell line

Project description:Breast tumors often exhibit intratumoral heterogeneity. We hypothesized that phenotypically distinct clonal subpopulations in heterogeneous tumors may affect overall tumor morphology and cancer cell invasion. In order to study heterogeneous tumors, we isolated individual cells from a parental 4T1 murine mammary carcinoma cell line and generated four clonal subpopulations (E1, E2, A, and M). To characterize these subpopulations, we examined gene expression by performing bulk RNA-sequencing.

2024-01-31 | GSE252177 | GEO

Expression data of 4T1 mammary cancer cells cultured in MSC-conditioned medium or co-cultured with MSCs

Project description:4T1 mammary cancer cells exihibit higher proliferation and metastatic ability when cultured in conditioned medium from mesenchymal stem cells . Also, co-implantation with MSCs, 4T1 tumors show higher tumor growth and metastasis. We used microarrays to detail the global programme of gene expression underlying the alteration of cell behaviour RNA was extrated from 4T1 cells cultured in MSC-CM or regular medium as well as 4T1 cells isolated from co-cultured with MSCs. Gene expression of these cell groups was acquired from Affymetrix microarray analysis using mouse 430 2.0 array chip.

2012-06-06 | E-GEOD-38251 | biostudies-arrayexpress

Single cell RNA-seq of 4T1 mammary tumors treated with Axitinib

Project description:To understand the effect of hypoxia on vasculogenic mimicry we treated mammary tumour dervied from parental 4T1 cells with vehicle or Axitinib. Tumors were analyzed by scRNA-seq and the expression of endothelial genes assessed in these data with and wihtout Axitinib treatment.

2023-07-26 | GSE230641 | GEO

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