Genomics

Dataset Information

0

TRIM33 switches off Ifnb1 transcription during the late phase of macrophage activation


ABSTRACT: Despite its importance during viral or bacterial infections, transcriptional regulation of the interferon-b gene (Ifnb1) in activated macrophages is only partially understood. Here we report that TRIM33 deficiency results in high-sustained expression of Ifnb1 at late stages of toll-like receptor-mediated activation in macrophages but not in fibroblasts. In macrophages, TRIM33 is recruited by PU.1 to a conserved region, Ifnb1 Control Element (ICE), located 15kb upstream of the Ifnb1 transcription start site. ICE constitutively interacts with Ifnb1 through a TRIM33-independent chromatin loop. At late phases of lipopolysaccharide activation of macrophages, TRIM33 is bound to ICE, regulates Ifnb1 enhanceosome loading, controls Ifnb1 chromatin structure, and represses Ifnb1 gene transcription by preventing recruitment of CBP/p300. These results characterize a previously unknown mechanism of macrophage-specific regulation of Ifnb1 transcription whereby TRIM33 is critical for Ifnb1 gene transcription shut down.

ORGANISM(S): Mus musculus

PROVIDER: GSE73322 | GEO | 2015/11/18

SECONDARY ACCESSION(S): PRJNA296598

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-43654 | biostudies-arrayexpress
2015-11-23 | GSE43654 | GEO
| PRJNA296598 | ENA
2019-07-26 | GSE124778 | GEO
2021-09-17 | GSE73448 | GEO
2020-10-09 | GSE137514 | GEO
2019-08-06 | GSE132922 | GEO
2019-08-06 | GSE117333 | GEO
2023-07-24 | PXD037854 | Pride
| E-MTAB-1441 | biostudies-arrayexpress