Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PLZF & SALL4 ChIP-seq in undifferentiated spermatogonia

ABSTRACT: Cultured THY1+ spermatogonia were used to reveal the genomic targets of PLZF and SALL4 in undifferentiated spermatogonia.

ORGANISM(S): Mus musculus

PROVIDER: GSE73390 | GEO | 2016/04/19

SECONDARY ACCESSION(S): PRJNA296869

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Identification of gene targets of the transcription factor SALL4 in undifferentiated spermatogonia

Project description:Sustained spermatogenesis in adult males and recovery of fertility following germ cell depletion are dependent on undifferentiated spermatogonia with self-renewal potential. We have previously demonstrated a critical role for the transcription factor Spalt-like 4 (SALL4) in spermatogonial differentiation. However, it remains unclear whether SALL4 has broader roles within the spermatogonial pool despite its ability to co-regulate genes with PLZF, a transcription factor required for undifferentiated cell maintenance. To identify genes regulated by SALL4 in the male germline, we established cultures of undifferentiated spermatogonia from a Sall4 inducible knockout mouse model. Cells were treated with vehicle (as control) or tamoxifen to induce gene deletion, then cells harvested and analysed by microarray to identify genes mis-expressed upon loss of SALL4.

2017-08-31 | GSE98991 | GEO

PLZF and DDX5 interacting proteins in spermatogonia

Project description:DDX5, or PLZF co-immunoprecipitation in lysates from cultured undifferentiated spermatogonia followed by identification of eluted proteins using mass spectrometry. IgG control IPs included.

2019-02-24 | MSV000083476 | MassIVE

The regulatory repertoire of PLZF and SALL4 in undifferentiated spermatogonia.

Project description: Not available

| S-EPMC4920160 | biostudies-literature

Regulation of gene expression in undifferentiated spermatogonia by DDX5

Project description:The maintenance of spermatogenesis in adult males is dependent on a population of mitotic germ cells with self-renewal potential known as undifferentiated spermatogonia. Regulation of undifferentiated spermatogonia function is dependent on transcriptional and post-transcriptional mechanisms. We have identified an essential role for the RNA helicase DDX5 in undifferentiated spermatogonia through generation of a UBC-CreERT2;Ddx5flox/flox mouse model that allows tamoxifen-dependent Ddx5 ablation. To identify genes regulated by DDX5, we generated lines of cultured undifferentiated spermatogonia from these mice and treated cells with tamoxifen (TMX) to induce Ddx5 knockout or vehicle (VEH) as a control, and performed RNA-sequencing analysis to compare these conditions.

2019-04-02 | GSE127275 | GEO

Analysis of gene expression heteroegeneity in adult undifferentiated spermatogonia [single-cell RNA-Seq]

Project description:The undifferentiated spermatogonial population of mouse testis is functionally heterogeneous and contains stem cells and committed progenitor cells. However, gene expression patterns marking these distinct cell fractions are poorly defined. Therefore, our aim was to profile gene expression of undifferentiated cells by RNA-Seq at the single cell level. Undifferentiated cells were therefore isolated from adult mouse testes based on expression of a Plzf/Zbtb16 gene reporter, which marks both stem and progenitor spermatogonia. Isolated cells were then processed using the 10X Chromium system and analysed by RNA-Seq. Our goal was to characterize distinct subsets of undifferentiated spermatogonia based on gene expression patterns.

2018-07-26 | GSE112880 | GEO

Androgen Promotes Differentiation of Promyelocytic Leukemia Zinc Finger (PLZF)+ Spermatogonia pool via Indirect Regulatory Pattern

Project description:We examined a regulatory role of the AR during the process of spermatogenesis. Using a SSCs-Sertoli cells co-culture system, we demonstrated that androgen negatively regulated Plzf in SSCs that co-exist with Sertoli cells. In addition, we identified Gata2 as a target of AR in Sertoli cells, and subsequently observed that Wilms tumor 1 (WT1) and β1-integrin as two putative intermediate molecules to transfer the differentiation signals to SSCs. This signal pathway was further verified using androgen pharmacological deprivation mice model. These results demonstrate a regulatory pattern of androgen in SSCs niche, that androgen turns off the stemness maintenance switch PLZF in undifferentiated spermatogonia populations to promote spermatogenesis in an indirect way via multiple steps of signal transduction.

2019-02-28 | E-MTAB-7129 | biostudies-arrayexpress

Mus musculus

Project description:PLZF & SALL4 ChIP-seq in undifferentiated spermatogonia

| PRJNA296869 | ENA

Expression profiles of in vitro cultured murine undifferentiated and differentiating spermatogonia

Project description:Total RNA from in vitro cultured murine undifferentiated and differentiating spermatogonia (by retinoic acid induction) were collected for RNA-seq

2020-05-15 | GSE150583 | GEO

DAZL targets in undifferentiated spermatogonia

Project description:DAZL targets in undifferentiated spermatogonia

| PRJNA605338 | ENA

Gene expression in undifferentiated spermatogonia

Project description:Gene expression in undifferentiated spermatogonia

| PRJNA605339 | ENA