Dataset Information

Total RNA sequencing of ovary tissues at 49 days post coitus in Large White and Meishan pigs.

ABSTRACT: We collected the ovary samples at 49 days post coitus in Large White and Meishan adult female pigs, identified the differentially expressed protein coding genes and long non-coding RNAs between these two breeds. three individuals of each breed were harvested as biological replicates. Overall design: Identification of differentially expressed protein-coding genes and long non-coding RNAs in ovary at 49 days post coitus between Large White and Meishan pigs.

INSTRUMENT(S): Illumina HiSeq 2500 (Sus scrofa)

SUBMITTER: Zhonglin Tang

PROVIDER: GSE73593 | GEO | 2017-06-12

SECONDARY ACCESSION(S): PRJNA297376

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	GSE73593_RAW.tar	Raw
	filelist.txt	Txt

Items per page:

1 - 2 of 2

Publications

Comprehensive analysis of long non-coding RNAs highlights their spatio-temporal expression patterns and evolutional conservation in Sus scrofa.

Tang Zhonglin Z Wu Yang Y Yang Yalan Y Yang Yu-Cheng T YT Wang Zishuai Z Yuan Jiapei J Yang Yang Y Hua Chaoju C Fan Xinhao X Niu Guanglin G Zhang Yubo Y Lu Zhi John ZJ Li Kui K

Scientific reports 20170224

Despite modest sequence conservation and rapid evolution, long non-coding RNAs (lncRNAs) appear to be conserved in expression pattern and function. However, analysis of lncRNAs across tissues and developmental stages remains largely uncharacterized in mammals. Here, we systematically investigated the lncRNAs of the Guizhou miniature pig (Sus scrofa), which was widely used as biomedical model. We performed RNA sequencing across 9 organs and 3 developmental skeletal muscle, and developed a filteri ...[more]

PMID: 28233874

Similar Datasets

Project description:Time-dependent expression of functional proteins in fetal ovaries is important to understand the developmental process of the ovary. This study was carried out to enhance our understanding of the developmental process of porcine fetal ovaries and to better address the differences in fetal ovary development of local and foreign pigs. The objective of the present study is to test the expression of key proteins that regulate the growth and development of fetal ovaries in Meishan and Yorkshire porcine breeds by using proteomics technology. Six Meishan and 6 Yorkshire pregnant gilts were used in this experiment. Fetal ovaries were obtained from Yorkshire and Meishan gilts on days 55 and 90 of the gestation period. Using 2D-DIGE (two dimensional-difference in gel electrophoresis) analysis, the results showed that there are about 1551 and 1400 proteins in gilt fetal ovaries on days 55 and 90, respectively of the gestation. Using MALDI TOF-TOF MS analysis, 27 differentially expressed proteins were identified in the fetal ovaries of the 2 breeds on day 55 of gestation, and a total of 18 proteins were identified on day 90 of gestation. These differentially expressed proteins were involved in the regulation of biological processes (cell death, stress response, cytoskeletal proteins) and molecular functions (enzyme regulator activity). We also found that alpha-1-antitrypsin, actin, vimentin, and PP2A proteins promote the formation of primordial follicles in the ovaries of Yorkshire pigs on day 55 of gestation while low expression heat shock proteins and high expression alpha-fetoproteins (AFP) may promote Meishan fetal ovarian follicular development on day 90 of gestation. These findings provide a deeper understanding of how reduced expression of heat shock proteins and increased expression of AFP can significantly reduce the risk of reproductive disease in obese Meishan sows. Our study also shows how these proteins can increase the ovulation rate and may be responsible for the low reproductive efficiency reported in other obese breeds. The ovarian developmental potential was found to be greater in Meishan pigs than in Yorkshire pigs.