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U937 miR-194-5p (UmiR-194-5p) vs U937 miR-194-5p (UmiR-194-5p) treated with SAHA (Vorinostat; suberoylanilide hydroxamic acid)

ABSTRACT: Transcriptional profiling of U937 miR-194-5p (UmiR-194-5p) vs U937 miR-194-5p (UmiR-194-5p) treated with SAHA (Vorinostat; suberoylanilide hydroxamic acid) for 24 h at 5uM concetration

ORGANISM(S): Homo sapiens

PROVIDER: GSE73648 | GEO | 2017/05/22

SECONDARY ACCESSION(S): PRJNA297482

REPOSITORIES: GEO

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U937 miR-194-5p (UmiR-194-5p) vs U937 miR-194-5p (UmiR-194-5p) treated with MS275 (SNDX 275;Entinostat)

Project description:Transcriptional profiling of U937 miR-194-5p (UmiR-194-5p) vs U937 miR-194-5p (UmiR-194-5p) treated with MS275 (SNDX 275;Entinostat) for 24 h at 5uM concetration

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U937 scramble (Usc) vs U937 miR-194-5p (UmiR-194-5p)

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Identification of miR-194-5p target genes in gastric cancer

Project description:Increasing studies report that miR-194-5p plays a tumor suppressor role in gastric cancer (GC). Previous studies have revealed that miR-194 inhibited gastric cancer progression through different pathways by affecting the expression level of different target genes. For example, miR-194 have been reported to be able to regulate the expression of FOXM1, NR2F2, BMI1, SDAD1, RBX1, KDM5B, ZEB1 and AKT2 etc. It suggested that miR-194 may play complicated roles in GC. To figure out the mechanism of miR-194' tumor suppressor role in GC, we performed RNA sequencing in two different GC cell lines. Our studies showed that miR-194 tends to regulated target genes by binding on their 3' untranslated regions with either 7-mer-A1 or 7-mer-m8 or 8-mer. Approximately 138 genes were downregulated in both SGC7901 and BGC823 cell lines that transfected with miR-194-5p mimics compared to negative control siRNAs. Most of the downregulated genes have not reported yet.

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Project description:Transcriptional profiling of U937 scramble vs shHDAC2 before and after SAHA treatment at 5ÂµM concentration for 6 and 24 hours. Different Experimental Conditions: U937 scramble (U937 trasfected with empty vector) vs shHDAC2 (U937 trasfected with shHDAC2 vector), untreated and treated with SAHA at 5 ÂµM concentration for 6h and 24h. Biological replicates: 2 for each sample, independently grown and harvested at 6 and 24 hours. One replicate per array.

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Downregulation of miR-194-5p induces paclitaxel resistance in ovarian cancer cells by altering MDM2 expression.

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