Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differential gene expression in the mesenteric fat among crossbred beef steers with divergent gain and feed intake phenotypes

ABSTRACT: Steer mesenteric fat transcriptome. Evaluation of the naturally occurring transcriptome variation in mesenteric fat among beef steers with divergent gain and feed intake phenotypes.

ORGANISM(S): Bos taurus

PROVIDER: GSE73699 | GEO | 2015/11/30

SECONDARY ACCESSION(S): PRJNA297687

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Differential gene expression in the liver among crossbred beef steers with divergent gain and feed intake phenotypes

Project description:Steer liver transcriptome Evaluation of the naturally occurring transcriptome variation in liver among beef steers with divergent gain and feed intake phenotypes.

2016-03-31 | GSE75700 | GEO

Differential gene expression in the spleen among crossbred beef steers with divergent gain and feed intake phenotypes.

Project description:Steer spleen transcriptome Evaluation of the naturally occurring transcriptome variation in the spleen among beef steers with divergent gain and feed intake phenotypes.

2015-10-30 | GSE73261 | GEO

Differential gene expression in the duodenum, jejunum, and ileum among crossbred beef steers with divergent gain and feed intake phenotypes.

Project description:Steer small intestine transcriptome Evaluation of the naturally occurring transcriptome variation among beef steers with divergent gain and feed intake phenotypes.

2015-09-19 | GSE73159 | GEO

Hepatic Gene Expression Profiles of Growing Beef Steers Grazing High vs. Low Endophyte-Infected Tall Fescue Grass

Project description:Neotyphodium coenophialum is an endophytic fungus that infects most tall fescue (Festuca arundinacea) pastures that are commonly used in animal grazing systems in the United States. Beef cattle grazing such pastures are impaired in health and production performance, resulting in a large economic loss in US food-animal production systems. Based on the clinical symptoms and laboratory analyses of blood, it was hypothesized that such affected cattle display liver-specific changes in the expression of gene transcripts that are associated with the metabolic enzymes and transporters critical for beef health and performance. Microarray analysis using the GeneChip Bovine Genome Array (Affymetrix, Inc., Santa Clara, CA) was conducted to determine if grazing endophyte-infected tall fescue pastures affects the liver gene expression profiles of growing beef steers. Nineteen steers were assigned to graze either a low toxic endophyte tall fescue-mixed grass (LE treatment, 5.7 ha, n = 9) or a high toxic endophyte infected tall fescue (HE treatment, 5.7 ha, n = 10) pasture located in the University of Kentucky Agricultural Research Center. All steers had ad libitum access to fresh water and an industry standard mineral-vitamin supplement. 88 days grazing on pasture. Approximately 2 g of tissue from the right lobe of the liver of each steer were collected for RNA extraction and microarray analysis.

2010-11-30 | E-GEOD-23894 | biostudies-arrayexpress

Identification of key pathways associated with residual feed intake of beef cattle

Project description:The biological mechanisms associated with the residual feed intake in ruminants have been harnessed immensely via transcriptome analysis of liver and ruminal epithelium, however, this concept has not been fully explored using whole blood. We applied whole blood transcriptome analysis and gene set enrichment analysis to identify key pathways associated with divergent selection for low or high RFI in beef cattle. A group of 56 crossbred beef steers (average BW = 261.3 ± 18.5 kg) were adapted to a high-forage total mixed ration in a confinement dry lot equipped with GrowSafe intake nodes for period of 49 d to determine their residual feed intake (RFI). After RFI determination, weekly whole blood samples were collected three times from beef steers with the lowest RFI (most efficient; low-RFI; n = 8) and highest RFI (least efficient; high-RFI; n = 8). Prior to RNA extraction, whole blood samples collected were composited for each steer. Sequencing was performed on an Illumina NextSeq2000 equipped with a P3 flow. Gene set enrichment analysis (GSEA) was used to analyze differentially expressed gene sets and pathways between the two groups of steers. Results of GSEA revealed pathways associated with metabolism of proteins, cellular responses to external stimuli, stress, and heat stress were differentially inhibited (false discovery rate (FDR) < 0.05) in high-RFI compared to low-RFI beef cattle, while pathways associated with binding and uptake of ligands by scavenger receptors, scavenging of heme from plasma, and erythrocytes release/take up oxygen were differentially enriched (FDR < 0.05) in high-RFI, relative to low-RFI beef cattle. Taken together, our results revealed that beef steers divergently selected for low or high RFI revealed differential expressions of genes related to protein metabolism and stress responsiveness.

2022-04-07 | GSE198068 | GEO

Hepatic Gene Expression Profiles of Growing versus Finished Beef Steers

Project description:Growing and finishing phases are two important animal production stages, which differ fundamentally in compositional growth. However, the physiological mechanisms altered concomitantly with the shift in whole-body compositional gain as cattle fatten (growing vs. finished steers), are poorly understood. Microarray analysis using the Bovine Gene 1.0 ST Array was conducted to determine shifts in hepatic genomic expression profiles of growing vs. finishing beef steers. The specific overall hypothesis tested was that genes involved in amino acid, carbohydrate and lipid metabolism, antioxidant capacity and immune responses were differentially expressed in growing vs. finishing steers.

2017-12-11 | GSE107881 | GEO

Muscle in beef steers with low vs. high residual feed efficiency

2014-04-11 | GSE56705 | GEO

Muscle in beef steers with low vs. high residual feed efficiency

Project description:Transcriptional profiling of bovine skeletal muscle comparing metabolically efficient steers to metabolically inefficient steers to ascertain what expression differences can be found in muscle tissue that might help explain differences in this economically significant phenotype. Two condition experiment, efficient versus inefficient animals. 8 efficient and 8 inefficient samples were used representing 16 unique individual animals.

2014-04-11 | E-GEOD-56705 | biostudies-arrayexpress

MicroRNA expression in subcutaneous and visceral fat of beef steers fed different high or low fat diets

Project description:MicroRNAs are a class of molecular regulators found to participate in numerous biological processes, including adipogenesis. However, whether dietary changes impact on microRNA (miRNA) in ruminants has not been reported. Therefore, this study aimed to evaluate the dietary effect on miRNA expression in subcutaneous (backfat) and visceral fat depots (perirenal fat) from beef steers fed with different diets containing high or low fat levels. Fat tissues were collected from 16 Hereford x Aberdeen Angus cross bred steers (15.5 month old) fed high fat diet (5.85% fat, n=8) or control diet (1.95% fat, n=8). Total RNA from each animal was subjected to miRNA microarray analysis using a customized Agilent miRNA microarray containing 672 bovine miRNA probes. Expression of miRNAs was not equally detected under two diets; 169 miRNAs were commonly expressed while 75 were diet specific. The number of miRNAs detected per animal under high fat diet was higher than those fed control diet (p= 0.037 in subcutaneous fat and p= 0.002 in visceral fat).. Further qRT-PCR analysis confirmed that the expression of some miRNAs was highly influenced by diet (miR-19a, -92a, -92b, -101, -103, -106, -142-5p, and 296) or fat depot (miR-196a and -2454). Our results revealed that the miRNA expression can be influenced by types of fat tissues or diet, suggesting that miRNAs may regulate bovine adipogenesis when diet alters. In this study, a total of 32 adipose tissue samples were analyzed by microRNA microarrays, being 16 subcutaneus (backfat) and 16 visceral (perirenal fat) fat depots were collected from 16 animals (Control diet = 8) (High fat diet = 8).

2012-06-30 | E-GEOD-35012 | biostudies-arrayexpress

Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers.

Project description: Not available

| S-EPMC6946124 | biostudies-literature