Transcriptomics,Genomics

Dataset Information

46

Escherichia coli: untreated versus 5-azacytidine-treated expression profiles


ABSTRACT: DNA microarray experiments were used to compare gene expression profiles of untreated and 5-azacytidine treated Escherichia coli at both logarithmic phase and early stationary phase The goal was to determine the effect of cytosine DNA methylation loss on gene expression (5-azacytidine is a methylation inhibitor) Overall design: Untreated versus 5-azacytidine-treated cells at logarithmic phase and early stationary phase; 5 biological replicates both all experiments

INSTRUMENT(S): Agilent-020097 E. coli Gene Expression Microarray (Feature Number version)

SUBMITTER: Kevin T Militello  

PROVIDER: GSE73707 | GEO | 2016-09-24

SECONDARY ACCESSION(S): PRJNA297697

REPOSITORIES: GEO

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Publications

5-azacytidine induces transcriptome changes in Escherichia coli via DNA methylation-dependent and DNA methylation-independent mechanisms.

Militello Kevin T KT   Simon Robert D RD   Mandarano Alexandra H AH   DiNatale Anthony A   Hennick Stacy M SM   Lazatin Justine C JC   Cantatore Sarah S  

BMC microbiology 20160627 1


Escherichia coli K-12 strains contain DNA cytosine methyltransferase (Dcm), which generates 5-methylcytosine at 5'CCWGG3' sites. Although the role of 5-methylcytosine in eukaryotic gene expression is relatively well described, the role of 5-methylcytosine in bacterial gene expression is largely unknown.To identify genes that are controlled by 5-methylcytosine in E. coli, we compared the transcriptomes of cells grown in the absence and presence of the DNA methylation inhibitor 5-azacytidine. We o  ...[more]

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