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Identification of Drosophila Mid regulated genes in embryos

ABSTRACT: Drosophila midline(mid), encodes a T-box transcription factor that is required for cell fate specification and tissue morphogenesis during a series of developmental processes. However, little is known about the target of Mid. To identify the Mid regulated genes, we compared expression profiles of mRNA purified from wild-type and mid mutant embryos. Summary file is yw-mid.CHP.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE73802 | GEO | 2016/06/30

SECONDARY ACCESSION(S): PRJNA297945

REPOSITORIES: GEO

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Identification of Drosophila Mid regulated genes in embryos

Project description:Drosophila midline(mid), encodes a T-box transcription factor that is required for cell fate specification and tissue morphogenesis during a series of developmental processes. However, little is known about the target of Mid. To identify the Mid regulated genes, we compared expression profiles of mRNA purified from wild-type and mid mutant embryos. Summary file is yw-mid.CHP. yw and mid mutant embryos were selected at later stage of development for RNA extraction and hybridization on Affymetrix microarrays. We identified mid mutant embryos using CyOact-GFP balancer chromosome.

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Project description:To dissect the gene regulatory networks operating during soybean embryo development, we identified the binding sites genome-wide for transcription factor GLYMA.13G317000 in mid-maturation embryos using ChIP-seq.

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Identification of GLYMA.08G357600 binding sites in soybean mid-maturation embryos II.

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