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Genome-wide maps of chromatin state in ESET+/+ and ESET-/- DP thymocytes

ABSTRACT: We generated genome-wide H3K9me3-state maps of DP thymocytes purified from ESET+/+ and ESET-/- mice by using next generation sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE73831 | GEO | 2016/01/01

SECONDARY ACCESSION(S): PRJNA298080

REPOSITORIES: GEO

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Genome-wide maps of chromatin state in ESET+/+ and ESET-/- DP thymocytes

Project description:We generated genome-wide H3K9me3-state maps of DP thymocytes purified from ESET+/+ and ESET-/- mice by using next generation sequencing. Examination of H3K9 trimethylation in DP thymocytes purified from ESET+/+ and ESET-/- mice.

2016-07-03 | E-GEOD-73831 | biostudies-arrayexpress

Genome-wide analysis in ESET+/+ and ESET-/- thymocytes

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2016-01-01 | GSE73951 | GEO

Genome-wide analysis in ESET+/+ and ESET-/- thymocytes

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Project description:Review on the role of Bcl11b in thymus and periphery and impact on diseases RNA was extracted from DP thymocytes of bcl11bf/fCd4cre/tcra-/- and tcra-/- mice. Tcra-/- mice only have preselected DP thymocytes. Such mice were used to determine the role of Bcl11b before selection, considering the defective positive selection in bcl11bf/fcd4cre mice. RNA was isolated and submitted for library generation and microarray analysis to determine expression profile of bcl11b-/- preselected DP thymocytes.

2014-04-12 | E-GEOD-56714 | biostudies-arrayexpress

Mapping of H2A.Z nucleosomes in CD4/CD8 DP thymocytes

Project description:H2A.Z ChIP-Seq in CD4/CD8 DP thymocytes

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Gene expression profile of murine Flvcr-deleted and control CD4+CD8+ double-positive (DP) thymocytes

Project description:Genomewide microaarray analysis of murine DP thymocytes to determine the genes whose expression was altered by FLVCR loss Gene signatures from Flvcr+/+ DP thymocytes was compared to gene signatures from Flvcr-/- DP thymocytes

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Expression data from E protein deficient double-positive (DP) thymocytes

Project description:We wanted to test the role of mammalian E proteins E2A and HEB in the development of T cells. Using a conditional deletion system in which these proteins are deleted at the DP stage of T cell development, we compared DP thymocytes deficient for E2A, HEB or both to wild-type thymocytes

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Gene expression profile of murine Flvcr-deleted and control CD4+CD8+ double-positive (DP) thymocytes

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miRNA expression in PTEN-deficient premalignant DP thymocytes

Project description:T cell-specific deletion of PTEN induces premalignancy in CD4+ CD8+ (DP) immature T cells in the thymus, which progresses to the development of mature CD4+ T cell lymphomas in the lymph nodes and spleen. As part of a screen to identify factors that inhibit progression to malignancy, we compared miRNA expression in premalignant PTEN-deficient DP thymocytes versus wild-type controls. DP thymocytes were collected by cell sorting from three 9-week-old, premalignant T cell-specific PTEN-deficient mice (tPTEN-/-) and three littermate controls. miRNA expression was assessed relative to a reference pool generated from an equal mixture of all samples.

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