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Impact of adcR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Ni2+

ABSTRACT: Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type to D39 ΔadcR grown in CDM with 0.3 mM Ni2+

ORGANISM(S): Streptococcus pneumoniae D39

PROVIDER: GSE73851 | GEO | 2015/11/20

SECONDARY ACCESSION(S): PRJNA298224

REPOSITORIES: GEO

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Transcriptome response of the Streptococcus pneumoniae D39 wild-type to Ni2+

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grownin CDM with 0.5 mM Ni2+ to grown in CDM with 0 mM Ni2+

2015-11-20 | GSE73850 | GEO

Impact of adcR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Zinc

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔadcR to D39 wild-type grown in CDM plus 0.2mM Zn2+.

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D39 wild-type vs D39 ÎadcR In CDM with 0.3 mM Ni2+

Project description:By using the transcriptomic approach, we have elucidated the effect of Ni2+ on the global gene expression of S. pneumoniae D39 by identifying several differentially expressed genes/operons in the presence of a high extracellular concentration of Ni2+. The genes belonging to the AdcR regulon (adcRCBA, adcAII-phtD, phtA, phtB and phtE) and the PsaR regulon (pcpA, prtA and psaBCA) were highly upregulated in the presence of Ni2+. We have further studied the role of Ni2+ in the regulation of the AdcR regulon by using ICP-MS analysis, electrophoretic mobility shift assays and transcriptional lacZ-reporter studies, and demonstrate that Ni2+ is directly involved in the derepression of the AdcR regulon via the Zn2+-dependent repressor AdcR, and has an opposite effect on the expression of the AdcR regulon as compared to Zn2+. Comparison of the Streptococcus pneumoniae D39 wild-type vs D39 ÎadcR in CDM Plus 0.3 mM Ni2+ Two condition design comparison of Wild-type strain vs mutant strain including a dye swap

2015-11-20 | E-GEOD-73851 | biostudies-arrayexpress

Impact of naiR delation on the transcriptome of Streptococcus pneumoniae D39

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔnaiR to D39 wild type grown in CDM

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Impact of copY delation on the transcriptome of Streptococcus pneumoniae D39

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 copY-stop mutant to D39 wild-type grown in CDM without added copper.

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Impact of ccpA delation on the transcriptome of Streptococcus pneumoniae D39 in the presece of N-acetylglucosamine (NAG)

Project description:Transcriptome comparision of the Streptococcus pneumoniae D39 ccpA mutant to D39 wild-type grown in CDM + 0.5 % N-acetylglucosamine (NAG)

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Transcriptional response of Streptococcus pneumoniae D39 to Zinc

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in CDM Plus 0mM Zn2+ to grown in CDM plus 0.2 mM Zn2+.

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Transcriptome response of the Streptococcus pneumoniae D39 wild-type to Aspirin

Project description:Transcriptome Comparison of the Streptococcus pneumoniae D39 wild-type grown in CDM with 0 mM to 5mM Aspirin

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Transcriptome response of the Streptococcus pneumoniae D39 wild-type to cysteine

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