Transcriptomics,Genomics

Dataset Information

37

Combined hepatic transcriptional effects of gamma radiation and depleted uranium on Atlantic salmon (Salmo salar)


ABSTRACT: To study the short-term hepatic transcriptional changes and identify potential modes of action, Atlantic salmon (Salmo salar) were exposed to 70 mGy external gamma radiation, 0.25 mg/L depleted uranium and combination of these two. A combination of high density (60 k) custom oligonucleotide salmonid microarray and quantitative real-time reverse transcription polymerase chain reaction (qPCR) was used to perform gene expression analyses. Differentially expressed genes (DEGs) were determined using one-way analysis of variance (ANOVA) and Tukey posthoc tests. Functional enrichment analysis based on Gene Ontology (GO) was performed to link DEGs to their biological functions. The Salmo salar DEGs were further mapped to mammlian orthologs. By using ortholog DEGs, gene networks were built based on well-curated mammlian protein-protein interactions and pathways analyses were performed to link DEGs to specific toxicological/biological functions. The results obtained from microarray analysis were further verified using qPCR. Overall design: Juvenile Atlantic salmon were exposed to external gamma radiation emitted from a cobalt-60 source for 5h and waterborne depleted uranium for 48 h. Liver were samples and used for gene expression analysis.

INSTRUMENT(S): Agilent-035370 Salmonid_60k_Array_V1_060711 035285

SUBMITTER: You Song  

PROVIDER: GSE74012 | GEO | 2015-10-14

SECONDARY ACCESSION(S): PRJNA298742

REPOSITORIES: GEO

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Publications

Hepatic transcriptional responses in Atlantic salmon (Salmo salar) exposed to gamma radiation and depleted uranium singly and in combination.

Song You Y   Salbu Brit B   Teien Hans-Christian HC   Evensen Øystein Ø   Lind Ole Christian OC   Rosseland Bjørn Olav BO   Tollefsen Knut Erik KE  

The Science of the total environment 20160418


Radionuclides are a special group of substances posing both radiological and chemical hazards to organisms. As a preliminary approach to understand the combined effects of radionuclides, exposure studies were designed using gamma radiation (Gamma) and depleted uranium (DU) as stressors, representing a combination of radiological (radiation) and chemical (metal) exposure. Juvenile Atlantic salmon (Salmo salar) were exposed to 70mGy external Gamma dose delivered over the first 5h of a 48h period (  ...[more]

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