Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq analysis of 3 month old all Ret-positive (EGFP-labeled) dorsal root ganglia L345 or L6S1 neurons

ABSTRACT: The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (3 month old). To enrich for bladder projecting neurons L6S1 DRGs were FACS isolated. L3,4, 5 lumbar levels were also FACS isolated to aid in differential expression of Ret-positive populations from L6S1 and L345 and help enrich bladder Ret-positive neurons. The series further includes these populations from males and females to uncover sex based differences.

ORGANISM(S): Mus musculus

PROVIDER: GSE74138 | GEO | 2017/09/30

SECONDARY ACCESSION(S): PRJNA299153

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA-SEQ analysis of postnatal Ret-positive (EGFP-labeled) nociceptive (NaV1.8 population) dorsal root ganglia L345 or L6S1 neurons.

Project description:The goal of this set is to build expression data using FACS isolated Ret-positive cells (postnatal) harvested from a RetFloxedEGFP reporter mouse crossed with NaV1.8Cre mice to enrich for Ret nociceptor population. To enrich for bladder projecting neurons L6S1 DRGs were FACS isolated. L3,4, 5 lumbar levels were also FACS isolated to aid in differential expression of Ret-nociceptor population from L6S1 and L345 spinal DRG levels. The series further includes these populations from males and females to uncover sex based differences.

2017-09-30 | GSE77893 | GEO

RNA-SEQ analysis of E14.5 ALL Ret-positive (EGFP-labeled) FACSd dorsal root ganglia neurons.

Project description:The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (embryonic day E14.5). These can be used as a time point in comparative analysis of ontogeny of genes expressed at various developmental time points.

2017-09-30 | GSE77892 | GEO

RNA-SEQ analysis of E12.5 ALL Ret-positive (EGFP-labeled) FACSd dorsal root ganglia neurons

Project description:The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (embryonic day E12.5). These can be used as a time point in comparative analysis of ontogeny of genes expressed at various developmental time points.

2017-09-30 | GSE98592 | GEO

RNA-SEQ analysis of E18.5 ALL Ret-positive (EGFP-labeled) male or female dorsal root ganglia neurons.

Project description:The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse (embryonic day E18.5 male or female). These can be used as a time point in comparative analysis of ontogeny of genes expressed at various developmental time points.

2017-09-30 | GSE77891 | GEO

RNA-SEQ analysis of P2 ALL Ret-positive (EGFP-labeled) FACSd male or female dorsal root ganglia neurons

Project description:The goal of this set is to build expression data using FACS isolated Ret-positive cells harvested from a Ret-EGFP reporter mouse P2 (post-natal day 2 male or female). These can be used as a time point in comparative analysis of ontogeny of genes expressed at various developmental time points.

2024-02-17 | GSE98593 | GEO

RNA-SEQ E18.5 Ret-positive (EGFP-labeled) Nav1.8 dorsal root ganglia neurons.

Project description:The goal of this set is to build expression data using FACS isolated Ret-positiveNaV1.8 cells harvested from RetFloxedEGFP;NaV1.8Cre embryos (embryonic day E18.5 male or female). These can be used as a time point in comparative analysis of ontogeny of genes expressed at various developmental time points in Ret nociceptors.

2017-09-30 | GSE77894 | GEO

A novel FACS-based method to isolate enriched populations of neurons, astrocytes, and microglia

Project description:We developed a novel FACS-based method to isolate enriched populations of neurons, astrocytes, and microglia. Whole mouse brains were digested, and populations of neurons, astrocytes, and microglia were subsequently enriched by FACS using a combination of positive gating on cell-type specific markers and stringent negative selection against markers of oligodendrocytes and endothelial cells. We confirmed a strong enrichment of corresponding cell-type specific genes in isolated populations and a reduction in gene markers of endothelial cells and oligodendrocytes, demonstrating that highly pure populations of neurons, astrocytes, and microglia were obtained.

2020-09-16 | GSE139000 | GEO

Heppenstal_Ret_GFP

Project description:Transcription profiling of Ret-positive subpopulations of Dorsal Root Ganglia neurons in mouse

2016-08-01 | E-MTAB-1836 | biostudies-arrayexpress

Transcriptome analysis of RANK-positive and RANK-negative luminal progenitor subpopulations in the human breast

Project description:RANK-positive and RANK-negative luminal progenitor cells were isolated by FACS from histologically normal human breast tissue from wild-type human donors. RNA-seq gene expression profiling was used to find differentially expressed genes between the RANK-positive and RANK-negative cell populations.

2016-06-20 | GSE71203 | GEO

High throughput quantitative whole transcriptome analysis of CC10- B4+ and Krt5-CreERT2 - labeled distal lung cells

Project description:We used microfluidic single cell RNA-seq on adult isolated CC10-CreERT2 (negative) integrin beta4(pos) cells lung epithelial cells in order to determine the transcriptional profile of this putative progenitor population. CC10-CreERT2 / tdTomato (negative) integrin beta4(pos) cells were isolated by FACS, as were Krt5-CreERT2 / tdTomato (positive) cells. These cells were pooled and loaded onto the Fluidigm C1 device.

2014-11-17 | E-GEOD-61300 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data