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RNA-seq analysis of gliotactin (Gli)-knockdown Drosophila melanogaster posterior midguts enterocytes (EC)

ABSTRACT: The goal of this study is to analyze changes in gene expression produced in posterior midguts after knocking down Gli in enterocytes (EC) during 9 days. Gli was depleted from ECs using a drug-inducible version of the binary GAL4-UAS system in combination with UAS-GLI-RNAi. Targeted gene expression using the 5966GS-GAL4 driver is observed in adult ECs when flies are fed the progesterone analog RU486 (RU+); addition of ethanol is used as a control (RU-), providing cohorts of isogenic individuals with or without induction of the Gli-RNAi transgene.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE74168 | GEO | 2016/12/18

SECONDARY ACCESSION(S): PRJNA299215

REPOSITORIES: GEO

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