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Cryptococcus neoformans gene expression in minimal media of wild type (WT) and all1all2Δ mutant cells

ABSTRACT: To examine the comparative transcription profile of WT and all1all2Δ mutant cells in in vitro conditions to determine genes resposible for hyporvirulent phenotype of all1all2Δ mutant cells. Using transcriptional profiling, we determined that many genes involved in amino acid transport were differentially transcribed between the wild type and the all1all2Δ mutant strain. The genes differentially expressed in double mutant of ALL1 ALL2 did not overlap with ALL1 and ALL2 comparative transcriptomes with RC2 (WT).

ORGANISM(S): Cryptococcus neoformans

PROVIDER: GSE74218 | GEO | 2015/12/09

SECONDARY ACCESSION(S): PRJNA299667

REPOSITORIES: GEO

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Cryptococcus neoformans gene expression in minimal media of wild type (WT) and all1all2Î mutant cells

Project description:To examine the comparative transcription profile of WT and all1all2Î mutant cells in in vitro conditions to determine genes resposible for hyporvirulent phenotype of all1all2Î mutant cells. Using transcriptional profiling, we determined that many genes involved in amino acid transport were differentially transcribed between the wild type and the all1all2Î mutant strain. The genes differentially expressed in double mutant of ALL1 ALL2 did not overlap with ALL1 and ALL2 comparative transcriptomes with RC2 (WT). For arrays, total RNA was isolated from WT and all1all2Î mutant using Qiagen RNeasy Kit. For RNA extraction, WT and all1all2Î mutant were grown in minimal media. Cells were grown for overnight, diluted 1:100 and then again grown overnight or up to an OD of 0.6-0.7 with agitation (150rpm) at 37Â°C.

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Project description:To examine the comparative transcription profile of WT and all2Δ mutant cells in in vitro conditions to determine genes responsible for hypervirulent phenotype of all2Δ cells. Using transcriptional profiling, we determined that many genes involved in transport were differentially transcribed between the wild type and the mutant strain. With the help of further experiments we could rule out that ALL2 has a unique function in maintaining the intracellular pH under acidic conditions.

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Project description:To examine the comparative transcription profile of WT and ALL1 mutant cells in in vitro conditions to determine genes resposible for hypervirulent phenotype of ALL1 mutant cells. Using transcriptional profiling, we determined that many genes involved in oxidation reduction processes and iron homeostasis were differentially transcribed between the wild type and the mutant strain For arrays, total RNA was isolated from WT and ALL1 mutant using Qiagen RNeasy Kit. For RNA extraction, Wt and ALL1 mutant were grown in two different culture medium conditions including minimal media and YPD broth. Cells were grown for overnight, diluted 1:100 and then again grown overnight or up to an OD of 0.6-0.7 with agitation (150rpm) at 37°C.

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Cryptococcus neoformans gene expression in minimal media of wild type, ALL2 mutant, and ALL1ALL2 mutant cells

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Project description:C. neoformans gene expression in DMEM of WT and rim101 mutant cells

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Project description:We wanted to find out the transcriptomic response of growth-arrested Cryptococcus yeast cells when initially placed in environments in which they could reactivate or "wake up", focusing on the difference between a rich microbial media (YPD) and a common cell culture media, RPMI + 10% heat-inactivated fetal calf serum. Please contact Edward.Wallace@ed.ac.uk if you use this data and ask for citation information; we are currently (August 2020) working on a publication.

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