Transcriptomics,Genomics

Dataset Information

146

PAPTRAP


ABSTRACT: We report the mRNAs bound to ribosomes in peripheral astrocyte processes, thus suggesting local translation in astrocytes. Overall design: Isolation of a synaptoneurosome fraction from mouse cortex in which Astrocyte ribosomes were labeled with eGFP. Immunoprecipitation of GFP from this fraction to obtain astrocyte ribosomes away from the cell body. Performed RNA seq on these purified ribosomes and their bound mRNAs.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Joseph D Dougherty 

PROVIDER: GSE74456 | GEO | 2017-06-02

SECONDARY ACCESSION(S): PRJNA300571

REPOSITORIES: GEO

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Publications

Astrocytes locally translate transcripts in their peripheral processes.

Sakers Kristina K   Lake Allison M AM   Khazanchi Rohan R   Ouwenga Rebecca R   Vasek Michael J MJ   Dani Adish A   Dougherty Joseph D JD  

Proceedings of the National Academy of Sciences of the United States of America 20170424 19


Local translation in neuronal processes is key to the alteration of synaptic strength necessary for long-term potentiation, learning, and memory. Here, we present evidence that regulated de novo protein synthesis occurs within distal, perisynaptic astrocyte processes. Astrocyte ribosomal proteins are found adjacent to synapses in vivo, and immunofluorescent detection of peptide elongation in acute slices demonstrates robust translation in distal processes. We have also developed a biochemical ap  ...[more]

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