Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons Depends on Lhx2 [OmpCre:Lhx2]

ABSTRACT: Conditional deletion of Lhx2, and to a lesser extent, Emx2 in olfactory neurons alters odorant receptor expression frequency. This series describes 1 of the 5 array experiments. GeneChip microarrays were used to measure odorant receptor mRNAs and test whether their abundance was affected in conditional knockout mice

ORGANISM(S): Mus musculus

PROVIDER: GSE74522 | GEO | 2016/11/21

SECONDARY ACCESSION(S): PRJNA300654

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons Depends on Lhx2 [123Cre:Lhx2]

Project description:Conditional deletion of Lhx2, and to a lesser extent, Emx2 in olfactory neurons alters odorant receptor expression frequency. GeneChip microarrays were used to measure odorant receptor mRNAs and test whether their abundance was affected in knockout mice

2016-11-21 | GSE74524 | GEO

Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons Depends Lhx2 [OmpCre:Emx2]

2016-11-21 | GSE74523 | GEO

Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons Depends on Lhx2 [123Cre:Emx2]

2016-11-21 | GSE74525 | GEO

Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons Depends on Lhx2 [123cre:double knockout]

2016-11-21 | GSE74526 | GEO

Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons Depends on Lhx2

Project description:Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons Depends on Lhx2

| PRJNA300649 | ENA

Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons Depends on Lhx2

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-11-21 | GSE74527 | GEO

Silencing of odorant receptor gene expression by G protein M-NM-2M-NM-3 signaling ensures the expression of one odorant receptor per olfactory sensory neuron

Project description:Olfactory sensory neurons express just one out of a possible ~1000 odorant receptor genes, reflecting an exquisite mode of gene regulation. In one model, once an odorant receptor is chosen for expression, other receptor genes are suppressed by a negative feedback mechanism, ensuring a stable functional identity of the sensory neuron for the lifetime of the cell. The signal transduction mechanism subserving odorant receptor gene silencing remains obscure, however. Here we demonstrate in the zebrafish that odorant receptor gene silencing is dependent on receptor activity. Moreover, we show that signaling through G protein M-NM-2M-NM-3 subunits is both necessary and sufficient to suppress the expression of odorant receptor genes, and likely acts through histone methylation to maintain the silenced odorant receptor genes in transcriptionally inactive heterochromatin. These results provide new insights linking receptor activity with the epigenetic mechanisms responsible for ensuring the expression of one odorant receptor per olfactory sensory neuron. Total 6 samples were analyzed-3 controls & 3 samples

2013-12-16 | E-GEOD-53334 | biostudies-arrayexpress

Silencing of odorant receptor gene expression by G protein βγ signaling ensures the expression of one odorant receptor per olfactory sensory neuron

Project description:Olfactory sensory neurons express just one out of a possible ~1000 odorant receptor genes, reflecting an exquisite mode of gene regulation. In one model, once an odorant receptor is chosen for expression, other receptor genes are suppressed by a negative feedback mechanism, ensuring a stable functional identity of the sensory neuron for the lifetime of the cell. The signal transduction mechanism subserving odorant receptor gene silencing remains obscure, however. Here we demonstrate in the zebrafish that odorant receptor gene silencing is dependent on receptor activity. Moreover, we show that signaling through G protein βγ subunits is both necessary and sufficient to suppress the expression of odorant receptor genes, and likely acts through histone methylation to maintain the silenced odorant receptor genes in transcriptionally inactive heterochromatin. These results provide new insights linking receptor activity with the epigenetic mechanisms responsible for ensuring the expression of one odorant receptor per olfactory sensory neuron.

2013-12-16 | GSE53334 | GEO

Lhx2 Determines Odorant Receptor Expression Frequency in Mature Olfactory Sensory Neurons.

Project description: Not available

| S-EPMC5086798 | biostudies-literature

Chromatin State and Binding of Lhx2 and Ebf in Olfactory Sensory Neurons

Project description:The monoallelic expression of olfactory receptor (OR) genes is governed by the formation of large domain of heterochromatin over OR clusters and by enhancer elements embedded within these heterochromatic domains. Here we show that OR enhancers are bound by Ebf and Lhx2 in mature olfactory sensory neurons (OSNs), and that in the same cell population these sites are flanked by a histone modification characteristic of active enhancers (H3K7ac) as well as modifications characteristic of heterochromatin (H3K9me3 and H3K79me3). We use ATAC-Seq to show that the pattern of OR enhancer accessibility in subpopulations of mOSNs that express a specific OR gene is very similar to the pattern in mOSNs as a whole. Finally, we perturb Ebf and Lhx2 in mOSNs by conditionally deleting Lhx2 or by expressing an Ebf-Lhx2 fusion protein. We profile the effect of these perturbations on OR gene expression by RNA-seq and on chromatin state by ChIP-Seq and ATAC-Seq.

2017-09-22 | GSE93570 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data