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Serum Conditions Do Not Abolish The Tumorigenicity of Glioma Stem Cells

ABSTRACT: The subset of GBM patient samples gives rise to adherent cultures even in sphere culture conditions. Most samples in this subset are tumorigenic and exhibit a hybrid expression profile when tested with the marker panel. Cultures from these samples have a predominantly mesenchymal character based on substrate adherence, morphology, differentiation potential and gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE74571 | GEO | 2016/07/21

SECONDARY ACCESSION(S): PRJNA300852

REPOSITORIES: GEO

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Serum Conditions Do Not Abolish The Tumorigenicity of Glioma Stem Cells

Project description:The subset of GBM patient samples gives rise to adherent cultures even in sphere culture conditions. Most samples in this subset are tumorigenic and exhibit a hybrid expression profile when tested with the marker panel. Cultures from these samples have a predominantly mesenchymal character based on substrate adherence, morphology, differentiation potential and gene expression. Total RNA isolated from glioblastoma stem cells (GSC) cultured as spheres was compared to that from adherent GSCs cultured in sphere culture conditions that exhibited both GSC and mesenchymal properties.

2016-07-21 | E-GEOD-74571 | biostudies-arrayexpress

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