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In(1)BM2(reinverted) males: reared at room temperature vs. Cold shock treatment

ABSTRACT: Cold exposure leads to alteration in the structure of the male sex chromosome of the mutant strain In(1)BM2(reinverted). Genome wide expression profiling was used to identify candidate genes involved in the expression of this phenotype. Adult male flies were exposed to cold shock at 12±1°C for 4 hours and the differentially expressed genes in the strain was compared to similarly exposed wild type Oregon R males. The microarray data was further validated using real time PCR.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE74673 | GEO | 2015/11/05

SECONDARY ACCESSION(S): PRJNA301143

REPOSITORIES: GEO

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In(1)BM2(reinverted) males: reared at room temperature vs. Cold shock treatment

Project description:Cold exposure leads to alteration in the structure of the male sex chromosome of the mutant strain In(1)BM2(reinverted). Genome wide expression profiling was used to identify candidate genes involved in the expression of this phenotype. Adult male flies were exposed to cold shock at 12Â±1Â°C for 4 hours and the differentially expressed genes in the strain was compared to similarly exposed wild type Oregon R males. The microarray data was further validated using real time PCR. Two-condition experiment, RT vs. CS flies. Biological replicates: 2 control replicates, 2 replicates exposed to cold shock.

2015-11-05 | E-GEOD-74673 | biostudies-arrayexpress

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Translatome analysis by Ribosome profiling of Streptomyces coelicolor A3(2) cold-shock response

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Gamma delta T cells isolated from spleen of cold-exposed rats against room-temperature placed control

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Transcriptome of Anthurium andraeanum under cold stress

Project description:The transcriptome of leaves from two Anthurium cultivars, Oregon (Elegang, E, cold tolerant) and Fantasy love (Menghuan, MH, cold sensitive) under cold stress were sequenced by Illumina Novaseq™ 6000. Sequencing generated a total of 129.44 Gb of raw reads and an average of 7.19 Gb of reads for each sample. The analysis showed differences of transcriptomes between the control and the cold treatment samples. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles inAnthurium cold tolerance.Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the cold stress response of A. andraeanum.

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Expression Profiles of miRNAs in brown fat of mice at room temperature and cold exposure (8C)

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Establishing the conditions required to initiate a cold-shock response in Streptomyces coelicolor

Project description:RNA-seq analysis of wild type Streptomyces coelicolor A3(2) MT1110 to examine global transcriptional profile change when exposed to cold shock from 30°C to 10°C in rich liquid medium and from 30°C to 4°C on minimal solid medium.

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Transcriptomics of rapid cold-hardening and cold shock recovery in Sarcophaga bullata

Project description:The ability to rapidly respond to changes in temperature is critical for insects and other ectotherms living in variable environments. In a physiological process termed rapid cold-hardening (RCH), exposure to non-lethal low temperature allows many insects to significantly increase their cold tolerance in a matter of minutes to hours. Additionally, there are rapid changes in gene expression and cell physiology during recovery from cold injury, and we hypothesize that RCH may modulate some of these processes during recovery. In this study, we used a cDNA microarray to examine the molecular mechanisms of RCH and cold shock (CS) recovery in the flesh fly, Sarcophaga bullata. With our custom 2-color array, we measured expression of ~15,000 ESTs during RCH and during recovery from cold shock. Surprisingly, no transcripts were upregulated during RCH, and likewise, RCH had a minimal effect on the transcript signature during recovery from cold shock. However, during recovery from cold shock, we observed differential expression of ~1,400 ESTs, including a number of heat shock proteins, cytoskeletal components, and genes from several cell signaling pathways. Several gene pathways correlated well with metabolomics data, indicating that coordinated changes in gene expression and metabolism contribute to recovery from cold shock.

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