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Expression data from stably-transfected KYSE150, transfected with shlncRNA625 or shscramble

ABSTRACT: To delineate the putative biological functions for lncRNA625, We performed expression profile from stably-transfected KYSE150 transfected with shlncRNA625 or shscramble for functions of lncRNA625 in ESCC

ORGANISM(S): Homo sapiens

PROVIDER: GSE74707 | GEO | 2015/11/10

SECONDARY ACCESSION(S): PRJNA301552

REPOSITORIES: GEO

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Expression data from stably-transfected KYSE150, transfected with shlncRNA625 or shscramble

Project description:To delineate the putative biological functions for lncRNA625, We performed expression profile from stably-transfected KYSE150 transfected with shlncRNA625 or shscramble for functions of lncRNA625 in ESCC Stably-transfected KYSE150, transfected with shlncRNA625 or shscramble, were collected and lysed in TRIzol (Life technologies). Microarray experiments were performed following the Affymetrix protocol at the Shanghai Biotechnology Corporation.

2015-11-10 | E-GEOD-74707 | biostudies-arrayexpress

ESCC KYSE150 cells: TPA treatment vs. control

Project description:Complete lncRNA and mRNA expression profile of ESCC KYSE150 cells and TPA-treatment KYSE150 cells

2024-02-01 | GSE167247 | GEO

Homo sapiens

Project description:Expression data from stably-transfected KYSE150, transfected with shlncRNA625 or shscramble

| PRJNA301552 | ENA

Expression data from EP300 was subjected to knockdown by siRNA interference

Project description:We knocked down EP300 and examined the expression of lncRNA625 target genes. Gene expression profiling of knockdown samples on cDNA microarrays indicated that EP300 affected expression of several lncRNA625 downstream target genes Stably-transfected KYSE150, transfected with shlncRNA625 or shscramble, were collected and lysed in TRIzol (Life technologies). Microarray experiments were performed following the Affymetrix protocol at the Shanghai Biotechnology Corporation.

2015-11-10 | E-GEOD-74742 | biostudies-arrayexpress

Expression data in human esophageal squamous carcinoma cell line KYSE150 with LOXL2 delta 72 over-expression

Project description:We have identified a new novel spliced variant of Lysyl Oxidase-like 2 (LOXL2), termed LOXL2 delta72, in human oesophageal carcinoma cells (ESCC). To explore the biological function of this variant, the cDNA microarrays was perform to assess the genes expression induced by its over-expression in ESCC KYSE150. We used microarrays to detail the global programmed of genes expression when LOXL2 delta72 over-expressed in KYSE150 cells compared with wild-type LOXL2.

2016-03-28 | GSE53645 | GEO

Next generation sequencing facilitates transcriptome analyses of mock and NCCRP1 transfected esophageal squamous cell carcinoma cell line KYSE150 cells

Project description:We obtained transcriptome profiling (RNA-seq) of human esophageal squamous cell carcinoma cell line KYSE150 stabley transfected clones with pIRES2-EGFP vector or human NCCRP1-expression vector by using next generation sequencing.

2019-09-26 | GSE87665 | GEO

lncRNA profiles enable prognosis prediction and subtyping for esophageal squamous cell carcinoma

Project description:AC007128.1, a long non-coding RNA, was upregulated in both ESCC tissues and cells, and associated with poor prognosis in ESCC. AC007128.1 can promote migration and invasion in ESCC cell lines. By RNA-sequencing analysis, we observed the significant changes of genes important in AC007128.1-depleted cells. We generated two KYSE150 cell lines stably transduced with either a sh-NC lentiviral construct or a shRNA lentiviral construct designed to target AC007128.1 RNA to investigate the consequences on the gene expression profile of AC007128.1 knockdown in KYSE150 cells.

2021-02-24 | GSE167345 | GEO

Gene expression data from 4T1 cells stably transduced with lentiviral vectors encoding a control shRNA (shscramble) or two shRNAs targeting TM4SF1 (shTM4SF1-1 and shTM4SF1-5) and from 4TO7 cells stably expressing with or without TM4SF1

Project description:Compare the gene expression profile between shscramble with shTM4SF1 knockingdown 4T1 cells and between 4TO7 cells stably expressing with or without TM4SF1 Compare the gene expression profile between shscramble with shTM4SF1 knockingdown 4T1 cells and between 4TO7 cells stably expressing with or without TM4SF1

2016-06-11 | E-GEOD-62730 | biostudies-arrayexpress

LNCaP prostate cancer cells stably transfected with shRNAs targeting ADRB2

Project description:Gene expression profiling of prostate adenocarcinoma LNCaP cells stably transfected with shRNAs targeting ADRB2 (shADRB2-2 and shADRB2-3) or control shRNA (shCtrl) incubated in 10% FCS.

2019-04-25 | GSE130204 | GEO

Project description:Compare the gene expression profile between shscramble with shTM4SF1 knockingdown 4T1 cells and between 4TO7 cells stably expressing with or without TM4SF1

2016-06-11 | GSE62730 | GEO

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