Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional Genomic Analyses of Hep-G2 Cell Line after FTO knockdown

ABSTRACT: To explore the molecular mechanism underlying glucose regulation by hepatic FTO, we used the human hepatocyte Hep-G2 cell line as an experimental platform and analyzed transcriptome changes following FTO knock-down.

ORGANISM(S): Homo sapiens

PROVIDER: GSE74791 | GEO | 2016/12/01

SECONDARY ACCESSION(S): PRJNA301528

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Functional Genomic Analyses of Hep-G2 Cell Line after FTO knockdown

Project description:To explore the molecular mechanism underlying glucose regulation by hepatic FTO, we used the human hepatocyte Hep-G2 cell line as an experimental platform and analyzed transcriptome changes following FTO knock-down.

2020-11-28 | GSE107411 | GEO

Functional Genomic Analyses of Hep-G2 Cell Line after FTO knockdown

Project description:Functional Genomic Analyses of Hep-G2 Cell Line after FTO knockdown

| PRJNA420031 | ENA

Transcriptomic analysis of of hepatocellular carcinoma cells Hep G2 overexpressing EFTUD2

Project description:We performed RNA-sequencing to detect the effects of overexpressing EFTUD2 in Hep G2 cells on gene expression.

2020-07-15 | GSE154438 | GEO

Homo sapiens strain:hep G2

Project description:Homo sapiens strain:hep G2 Transcriptome or Gene expression

| PRJNA273555 | ENA

Homo sapiens strain:iPSC of Hep G2

Project description:Homo sapiens strain:iPSC of Hep G2 Transcriptome or Gene expression

| PRJNA273617 | ENA

Functional Genomic Analyses of hepatic tissue after FTO knockout

Project description:To explore the molecular mechanism underlying glucose regulation by hepatic FTO, we analyzed transcriptome changes in hepatic tissue after FTO knockout.

2018-12-01 | GSE90687 | GEO

HepG2 Cell: Control vs. HGF treated

Project description:Transcription profile of HepG2 cells treated with hepatocyte growth factor and control cells Two condition experiment, Hep G2 vs. Hep G2-HGF. Biological replicates: 1 control and 1 HGF-treated (no replication)

2012-02-23 | E-GEOD-35994 | biostudies-arrayexpress

Transcriptomic analysis of of hepatocellular carcinoma cells Hep G2 overexpressing EFTUD2

Project description:Transcriptomic analysis of of hepatocellular carcinoma cells Hep G2 overexpressing EFTUD2

| PRJNA646263 | ENA

Quantitative Proteomics of Mouse Liver (FTO-KO,WT) in response to DEN injection

Project description:Single nucleotide polymorphisms in the FTO gene encoding a m6A demthylase are associated with obesity and cancer development. However, the functional role of FTO in the developemnt of progression of hepatocellular carcinoma (HCC) as a proteotypic obesity-associated cancer remains unclear. Here, we have generated mice with hepatic FTO deficiency (FTOL-KO) and subjected them to DEN induced HCC-development. FTOL-KO mice exhibit increased HCC burden. While control mice exhibit a dynamic regulation of FTO upon induction of liver damage, this response is abrogated in mice lacking FTO. Proteomic analyses revealed that liver damage-induced increases in FTO expression promotes m6A-demethylation of CUL4A reducing its protein expression. Functionally, knockdown of CUL4A restores the increased hepatocyte proliferation observed upon loss of FTO. Collectively, our study reveals a protective role for FTO-dependent dynamic m6A mRNA demethylation of CUL4A in the initiation of HCC development.

2020-11-03 | PXD018223 | Pride

Effect of FTO overexpression and knockdown on transcriptome of HEK293 cells

Project description:To gain insight into FTO function, we knocked down and overexpressed FTO in HEK293 cells.Genetrail analyses of expression profiles pointed to the RNA splicing and processing machinery. Intriguingly, using immunocytochemistry and confocal laser scanning microscopy, we observed strong enrichment of FTO in nuclear speckles and - to a lesser extent - in nucleoli, but not in other known nuclear bodies. We also studied RNA samples of Fto knockout and wild type mice with regard to content of methylated and unmethylated nucleosidesand observed that ratios of modified and unmodified uracil and adenine were different depending on the presence of FTO. Taken together, our data suggest that FTO is involved in RNA processing and modification. We used microarrays to investigate global gene expression changes depending on the level of FTO We compared FTO overexpressing and FTO depleted cells to cells with endogeneus level of FTO to determine global gene expression changes.

2012-11-13 | E-GEOD-33870 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data