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Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

ABSTRACT: We use RNA-Seq to explore the reaction norm of gene expression of larval Anopheles coluzzii and An. merus to a gradient of salinity.

ORGANISM(S): Anopheles coluzzii Anopheles merus

PROVIDER: GSE74819 | GEO | 2016/03/09

SECONDARY ACCESSION(S): PRJNA301602

REPOSITORIES: GEO

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Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

Project description:We use RNA-Seq to explore the reaction norm of gene expression of larval Anopheles coluzzii and An. merus to a gradient of salinity. After rearing in freshwater, separate pools of 18 h post-hatch larval instars of An. merus and An. coluzzii were briefly (6 h) exposed to one of six water salinities: 0%, 10%, 20%, 30%, 40%, or 50% the salinity of seawater. The last sample (G-50-RC) was split across 5 lanes for sequencing, resulting in total 10 fastq files associated with subparts G-50-RCa, G-50-RCb, G-50-RCc, G-50-RCd, G-50-RCe.

2016-03-09 | E-GEOD-74819 | biostudies-arrayexpress

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

Project description:We use RNA-Seq to investigate gene expression differences in response to saltwater exposure by the larval stages of the obligate freshwater mosquito Anopheles coluzzii (formerly An. gambiae "M" form), and the euryhaline An. merus. After rearing in freshwater, both young and old larval instars of each species were briefly (6 h) exposed to either saltwater (SW) or freshwater (FW) conditions to test the impact of salinity on mRNA levels.

2016-03-09 | E-GEOD-59632 | biostudies-arrayexpress

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

2016-03-09 | GSE59632 | GEO

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress.

Project description: Not available

| S-EPMC4877251 | biostudies-literature

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-03-09 | GSE74820 | GEO

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

Project description:Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

| PRJNA301602 | ENA

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress

Project description:Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress

| PRJNA301598 | ENA

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

Project description:Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

| PRJNA255780 | ENA

Salinity adaptation in the principal arboviral vector Aedes aegypti characterized by larval cuticle changes

Project description:Aedes aegypti, the principle global vector of arboviral diseases, has been widely regarded to only lay eggs and undergo preimaginal development in fresh water collections. Recent observations however show that it has adapted to develop in anthropogenic brackish water habitats of up to 50% sea water in coastal areas in different continents. This adaptation is characterised by greater salinity tolerance in adult oviposition preference, larvae and changes in sizes of anal papillae. The physiological basis for salinity tolerance in either Ae. aegypti or any of the known salinity-tolerant species of Anopheles malaria vectors is not established. To address this knowledge gap which is of fundamental biological interest and important for control of major diseases we performed RNAseq analysis of gut, anal papillae, and rest of the carcass of Ae. aegypti collected in the field from brackish water (BW) and fresh water habitats (FW) and then maintained as laboratory colonies in BW and FW respectively. We also examined the cuticle structure of larvae, pupae and adult BW and FW Ae. aegypti by microscopy and performed proteomic analysis of the shed cuticles of fourth instar larvae (L4) when they transformed into pupae. The results show that major changes in cuticle structure and composition characterize, and may be the principal factor that permits, the adaptation of Ae. aegypti to brackish water.

2021-04-07 | PXD018397 | Pride

Comparison of a resistant field Anopheles coluzzii population from Banfora, Burkina Faso to lab susceptible N'Gousso 2014

Project description:Field resistant Anopheles coluzzii were compared to the lab susceptible Anopheles coluzzii N'Gousso. The samples were collected in 2014 in Burkina Faso and show resistance to pyrethroid insecticides.

2018-07-29 | E-MTAB-6498 | biostudies-arrayexpress

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