Transcriptomics,Genomics

Dataset Information

166

RNA-seq profiling of mouse PGC (Mus musculus)


ABSTRACT: PGCs undergo two distinct stages of demethylation before reaching a hypomethylated ground state at E13.5. Stage 1 occurs between E7.25- E9.5 in which PGCs experience a global loss of cytosine methylation. However, discreet loci escape this global loss of methylation and between E10.5-E13.5, stage 2 of demethylation takes place. In this stage these loci are targeted by Tet1 and Tet2 leading to the loss of the remaining methylation and resulting in the epigenetic ground state. Our data shows that Dnmt1 is responsible for maintaining the methylation of loci that escape stage 1 demethylation, and that it functions in a UHRF1 independent manner. Our data further demonstrates that when these loci lose methylation prior to stage 2 it results in early activation of the meiotic program, which leads to precocious differentiation of the germ line resulting in a decreased pool of PGCs in the embryo and subsequent infertility in adult mice. Overall design: Examination of transcription of Mouse PGCs

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Pao-Yang Chen 

PROVIDER: GSE74938 | GEO | 2016-09-13

SECONDARY ACCESSION(S): PRJNA301971

REPOSITORIES: GEO

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Publications

Stage-Specific Demethylation in Primordial Germ Cells Safeguards against Precocious Differentiation.

Hargan-Calvopina Joseph J   Taylor Sara S   Cook Helene H   Hu Zhongxun Z   Lee Serena A SA   Yen Ming-Ren MR   Chiang Yih-Shien YS   Chen Pao-Yang PY   Clark Amander T AT  

Developmental cell 20160909 1


Remodeling DNA methylation in mammalian genomes can be global, as seen in preimplantation embryos and primordial germ cells (PGCs), or locus specific, which can regulate neighboring gene expression. In PGCs, global and locus-specific DNA demethylation occur in sequential stages, with an initial global decrease in methylated cytosines (stage I) followed by a Tet methylcytosine dioxygenase (Tet)-dependent decrease in methylated cytosines that act at imprinting control regions (ICRs) and meiotic ge  ...[more]

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