Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genes activated by s.a through the activity of phosphatidylinositol 4-kinase- Role of phosphatidylinositol 4-kinase

ABSTRACT: Cell suspension culture of Arabidopsis thaliana (ecotype Columbia) was treated by 250 uM salicylic acid and by two different concentrations of wortmannin (1 uM and 30 uM) for 4 hours. Keywords: dose response,treated vs untreated comparison

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE7495 | GEO | 2007/04/12

SECONDARY ACCESSION(S): PRJNA100491

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of Arabidopsis cell suspension cultures treated with salicylic acid or wortmannin

Project description:Cell suspension culture of Arabidopsis thaliana (ecotype Columbia) was treated by 250 mM salicylic acid and by two different concentrations of wortmannin (1 mM and 30 mM) for 4 hours.

2007-04-01 | E-MEXP-837 | biostudies-arrayexpress

edelfosine and wortmannin effect at 22°c and 4°c-PI4-kinase and phospholipase C signaling in cold response

Project description:rs09-04_plc_2 - edelfosine and wortmannin effect at 22°c and 4°c - Is there an overlap between PI4-kinase and phospholipase C signaling in cold response ? - Experiments were carried out with Columbia suspension cells. Cells were treated or not with edelfosine (PLC inhibitor) or wortmannin (PI4-kinase inhibitor). These agents were added 15 min before cold stress. ARN were extracted 4h after stress. Controls were made at 22°C. Keywords: treated vs untreated comparison

2010-08-01 | GSE19850 | GEO

edelfosine and wortmannin effect at 22°c and 4°c-PI4-kinase and phospholipase C signaling in cold response

2010-08-01 | E-GEOD-19850 | biostudies-arrayexpress

Difference in expression between SA- and mock-treated leaves 2 hours after application

Project description:Arabidopsis plants, ecotype Columbia, were sprayed with surfactant alone (0.01% Silwet) or surfactant and 1mM salicylic acid until all leaves were wet, 3 to 4 weeks after germination (before flowering). Leaves were harvested and frozen in liquid nitrogen. Three biological replicates were obtained over a period of 6 months. For each replicate, RNA was extracted using standard phenol/chloroform protocol and biotin-labeled cRNA was synthesized. cRNA was hybridized to 6 ATH1 GeneChip arrays. Cel files produced by the Affymetrix software were imported into R. Background subtraction, normalization and probe summaries were performed with the rma, quantile.normalization and median polish options of the rma function. Comparison of the SA- versus mock-treated tissue provides insight on the genes involved in systemic acquired resistance in Arabidopsis. Keywords: salicylic acid, Arabidopsis, stress response, defense,

2006-03-30 | GSE3984 | GEO

RNA-seq of Arabidopsis thaliana seedlings treated with MeJA or BTH

Project description:This study evaluates the transcriptome of Arabidopsis thaliana seedlings (Col-0 ecotype) treated with methyl jasmonate (MeJA) or with the salicylic acid analog benzothiadiazole (BTH).

2017-01-12 | GSE90077 | GEO

Transcription profiling of Arabidopsis suspension cells exposed to a range of abiotic treatments

Project description:Profiling the transcriptome of Arabidopsis suspension cells in response to a range of abiotic treatments. Experiment Overall Design: variable_1 = 3 h Experiment Overall Design: variable_2 = 12 h Experiment Overall Design: variable_3 = untreated, control Experiment Overall Design: variable_4 = hydrogen peroxide Experiment Overall Design: variable_5 = N2 air replacement Experiment Overall Design: variable_6 = chitin Experiment Overall Design: variable_7 = cold Experiment Overall Design: variable_8 = cysteine Experiment Overall Design: variable_9 = flagellen Experiment Overall Design: variable_10 = mannitol Experiment Overall Design: variable_11 = norflurazon Experiment Overall Design: variable_12 = oligomycin 0.125 uM Experiment Overall Design: variable_13 = oligomycin 1.25 uM Experiment Overall Design: variable_14 = roteonone Experiment Overall Design: variable_15 = salicylic acid 10 uM Experiment Overall Design: variable_16 = salicylic acid 100 uM Experiment Overall Design: variable_17 = chloramphenicol

2007-07-11 | E-GEOD-3709 | biostudies-arrayexpress

Functional convergence of growth responses to shade and warmth in Arabidopsis

Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of Arabidopsis thaliana Columbia-0 ecotype seedlings that were treated with two different light (white light or shade) and temperature (20ºC or 28ºC) conditions.

2021-08-09 | GSE162240 | GEO

Transcriptional characterization of Arabidopsis thaliana seeds treated with a red- or far red-light pulse

Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of imbibed Arabidopsis thaliana Columbia-0 ecotype seeds that were treated with a 20 min red or far red pulse. The red-light pulse induces germination.

2019-07-10 | GSE134019 | GEO

Regulation of the microsomal proteome by salicylic acid and deficiency of phosphatidylinositol‐4‐kinases β1 and β2 in Arabidopsis thaliana

Project description:Phosphatidylinositol-4-kinases β1 and β2 (PI4Kβ1/PI4Kβ2), which are responsible for phosphorylation of phosphoinositol to phosphatidylinositol-4-phosphate, have an important role in vesicular trafficking in plants. Moreover, PI4Kβ1/PI4β2 negatively regulate biosynthesis of phytohormone salicylic acid (SA). SA plays crucial role in plant immune responses and its endogenous concentration strongly affects plant transcriptome. In this study we were focused on the effect of the PI4Kβ1/PI4Kβ2 and SA on the abundance of membrane proteins. For this purpose, we performed proteomic analysis on isolated microsomal fractions from leaves of four Arabidopsis thaliana genotypes: wild type ecotype Columbia-0; double mutant impaired in function of PI4Kβ1/PI4Kβ2 (pi4kβ1/pi4kβ2) exhibiting high SA level; sid2 mutant with impaired SA biosynthesis depending on the isochorismate synthase 1 and triple mutant sid2/pi4kβ1/pi4kβ2. In total, we identified 4534 proteins from which 1696 proteins differed in abundance between the mutants and WT. We showed that SA has a big impact on membrane proteome, because among hundreds of the identified affected proteins typical proteins associated with SA triggered pathway occurred. Our data thus point out new connections between SA pathway and clathrin independent endocytosis (flotillins) and exocytosis/protein secretion (syntaxins, tertraspanin) to be investigated in future. In contrast to SA, presence/absence of PI4Kβ1/PI4Kβ2 itself affected only 27 proteins. Nevertheless, among them we identified CERK1, plasma membrane receptor for chitin. Although PI4Kβ1/PI4Kβ2 itself did not have strong impact on A. thaliana microsomal proteome, our data clearly shows that PI4Kβ1/PI4Kβ2 enhance the proteome changes when SA pathway is modulated in parallel

2021-02-24 | PXD019737 | Pride

Role of phospholipase D in salycilic acid signaling in Arabidopsis thaliana suspension cells

Project description:Our goal is to identify Salycilic Acid responsive genes dependent on PLD activation. - The experiment is done on Arabidopsis suspension cells, ecotype Columbia : Research of phospholipase D (PLD) activity implication in the response to SA. The use of primary alcohol, like N-butanol, makes possible to derive PLD activity towards the production of phosphatidylalcohol with the detriment of phosphatidic acid, the product of the PLD. Thus, in the presence of n-butanol, the response of the genes to SA dependent on phosphatidic acid will see their response disturbed. On the contrary, in the presence of tertiary butanol, the response of the genes should not be disturbed, tertiary alcohols not acting on the PLD. Keywords: treated vs untreated comparison

2007-11-29 | GSE9695 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data