Transcriptomics,Genomics

Dataset Information

175

RNA-seq analysis of chitin-triggered transcriptional reprogramming in the Arabidopsis mapkkk5 mutant


ABSTRACT: Perception of microbe-associated molecular patterns by host cell-surface pattern recognition receptors triggers a series of immune responses such as activation of mitogen-activated protein kinase (MAPK) cascades. In Arabidopsis thaliana, the receptor-like cytoplasmic kinase PBL27 interacts with the chitin receptor CERK1 and regulates chitin-induced MAPK activation. We found that the MAPK kinase kinase MAPKKK5 connects PBL27 to the downstream MAPK Kinases (MKK4/5) and MAPKs (MPK3/6). RNA-seq analysis using the mapkkk5 mutant indicated that MAPKKK5 plays important roles in chitin-induced transcriptional reprogramming. As far as we are aware, this is the first report pointing to the significance of a complete MAPK cascade in defense-associated transcriptional reprogramming in plants. Overall design: Arabidopsis Col-0 and mapkkk5-1 were treated with mock or chitin (40mM) for 3 hours. Three independent experiments were performed.

INSTRUMENT(S): Illumina HiSeq 2500 (Arabidopsis thaliana)

SUBMITTER: akira mine  

PROVIDER: GSE74955 | GEO | 2017-01-05

SECONDARY ACCESSION(S): PRJNA302003

REPOSITORIES: GEO

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Publications


Perception of microbe-associated molecular patterns by host cell surface pattern recognition receptors (PRRs) triggers the intracellular activation of mitogen-activated protein kinase (MAPK) cascades. However, it is not known how PRRs transmit immune signals to MAPK cascades in plants. Here, we identify a complete phospho-signaling transduction pathway from PRR-mediated pathogen recognition to MAPK activation in plants. We found that the receptor-like cytoplasmic kinase PBL27 connects the chitin  ...[more]

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