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On the Origin and Evolutionary Consequences of Gene Body DNA Methylation

ABSTRACT: In plants, CG DNA methylation is prevalent in the transcribed regions of constitutively expressed genes (“gene body methylation; gbM”), but the origin and function of gbM remain unknown. Here we report the discovery that Eutrema salsugineum, has lost gbM from its genome, the first known instance for a flowering plant. Of all known DNA methyltransferases, only CHROMOMETHYLTRANSFERASE 3 (CMT3) is missing from E. salsugineum, indicating that CMT3 may be required for the establishment of gbM. Detailed analyses of gene expression, the histone variant H2A.Z and various histone modifications in E. salsugineum and Arabidopsis thaliana epiRILs turned up no evidence in support of any role for gbM in regulating transcription or affecting the composition and modifications of chromatin over evolutionary time scales.

ORGANISM(S): Arabidopsis thaliana Eutrema salsugineum Brassica rapa Arabidopsis lyrata Capsella rubella Brassica oleracea

PROVIDER: GSE75071 | GEO | 2016/07/11

SECONDARY ACCESSION(S): PRJNA302602

REPOSITORIES: GEO

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