Genomics

Dataset Information

36

RNA-Seq: assessment of transcript level analysis tools


ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Overall design: Refer to individual Series

INSTRUMENT(S): [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]

SUBMITTER: Gilgi Friedlander 

PROVIDER: GSE75139 | GEO | 2016-04-26

SECONDARY ACCESSION(S): GSE71802PRJNA302625 GSE75138

REPOSITORIES: GEO

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Publications

Using Synthetic Mouse Spike-In Transcripts to Evaluate RNA-Seq Analysis Tools.

Leshkowitz Dena D   Feldmesser Ester E   Friedlander Gilgi G   Jona Ghil G   Ainbinder Elena E   Parmet Yisrael Y   Horn-Saban Shirley S  

PloS one 20160421 4


One of the key applications of next-generation sequencing (NGS) technologies is RNA-Seq for transcriptome genome-wide analysis. Although multiple studies have evaluated and benchmarked RNA-Seq tools dedicated to gene level analysis, few studies have assessed their effectiveness on the transcript-isoform level. Alternative splicing is a naturally occurring phenomenon in eukaryotes, significantly increasing the biodiversity of proteins that can be encoded by the genome. The aim of this study was t  ...[more]

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