Transcriptomics,Genomics

Dataset Information

39

HrcA Regulon in Listeria monocytogenes


ABSTRACT: The foodborne pathogen Listeria monocytogenes uses a number of transcriptional regulators, including the negative regulator HrcA, to control gene expression under different environmental conditions and in response to stress. Gene expression patterns of DhrcA stationary phase cells were compared to wt to identify hrcA-dependent genes. We identified 61 HrcA-dependent genes that showed significant expression ratios (adj. P < 0.05), with = 1.5-fold differential expression between ?hrcA and wt. Combined with microarray analysis, Hidden Markov Model searches show HrcA directly repress at least 8 genes. Keywords: Listeria monocytogenes, HrcA regulon, stationary phase Overall design: Independent RNA isolations were performed for each growth experiment (log phase cells). For wt vs. hrcA null mutant comparisons, three biological replicates were used in competitive whole-genome microarray experiments. For each set of hybridizations, RNA from the parent strain (10403S) was hybridized to RNA from the hrcA null mutant.

INSTRUMENT(S): CUFSL Listeria 6K oligonuclotide array v1.0

SUBMITTER: Sarita Raengpradub  

PROVIDER: GSE7517 | GEO | 2007-11-03

SECONDARY ACCESSION(S): PRJNA99417

REPOSITORIES: GEO

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Publications

Transcriptomic and phenotypic analyses suggest a network between the transcriptional regulators HrcA and sigmaB in Listeria monocytogenes.

Hu Yuewei Y   Oliver Haley F HF   Raengpradub Sarita S   Palmer M Elizabeth ME   Orsi Renato H RH   Wiedmann Martin M   Boor Kathryn J KJ  

Applied and environmental microbiology 20071026 24


Listeria monocytogenes HrcA and CtsR negatively regulate class I and III stress response genes, respectively, while sigma(B) positively regulates the transcription of class II stress response genes. To define the HrcA regulon and identify interactions between HrcA, CtsR, and sigma(B), we characterized newly generated L. monocytogenes DeltahrcA, DeltactsR DeltahrcA, and DeltahrcA DeltasigB strains, along with previously described DeltasigB, DeltactsR, and DeltactsR DeltasigB strains, using phenot  ...[more]

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