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Expression data from MCF-7 breast cancer cells treated with CNTs, ginsenosides (Rb1 and Rg1) and conjugates (Rb-CNTs and Rg-CNTs)

ABSTRACT: To investigate the effects of ginsenosides-CNTs conjugtaes on total expression profile of MCF-7 breast cancer cells, we conjugated ginsenoides Rb1 and Rg1 with multi-walled carbon nanotubes in a 5:1 ratio. Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in MCF-7 cells treated with ginsenoside-CNTs conjugates and compare it to the ginsenosides alone (Rb1 and Rg1)

ORGANISM(S): Homo sapiens

PROVIDER: GSE75570 | GEO | 2016/12/15

SECONDARY ACCESSION(S): PRJNA304617

REPOSITORIES: GEO

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Homo sapiens

Project description:Expression data from MCF-7 breast cancer cells treated with CNTs, ginsenosides (Rb1 and Rg1) and conjugates (Rb-CNTs and Rg-CNTs)

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Role of MicroRNA-214 in Ginsenoside-Rg1-induced Angiogenesis

Project description:MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional gene modulators. Ginsenoside-Rg1, one of the active components of ginseng, has been confirmed by us as an angiogenesis inducer. Using miRNA microarray analysis, a total of 15 (including miR-214) and 3 miRNAs were found to be down- or up-regulated by Rg1 in human umbilical vein endothelial cells (HUVEC), respectively. Since miR-214 is closely related to endothelial nitric oxide synthase (eNOS) and hence angiogenesis; its expression was further validated by qRT-PCR. We also investigated the role of miR-214 on eNOS expression and in tubulogenesis of HUVEC by transfection of specific miRNA inhibitor or precursor. Our results suggested that Rg1 can down-regulate miR-214 expression in HUVEC, leading to an increase in eNOS expression which can promote angiogenesis. This result signifies a new understanding towards how a simple natural compound can affect physiological changes through modulation of miRNA expression. The study is used to investigate the role of miRNA-214 in Rg1-induced human endothelial cells.

2010-08-05 | E-GEOD-17541 | biostudies-arrayexpress

Role of MicroRNA-214 in Ginsenoside-Rg1-induced Angiogenesis

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Rg1 improves motor recovery after spinal cord injury by suppressing astrocytic NLRP2 inflammasome via the TLR4–MyD88–NF-κB pathway

Project description:Spinal cord injury (SCI)-induced neuroinflammation and astrocyte pyroptosis severely impede motor functional recovery. Here, we report that ginsenoside Rg1 attenuates astrocyte pyroptosis by regulating the TLR4‑MyD88‑NF‑κB signaling axis, thereby promoting functional recovery in SCI rats. RNA‑seq combined with RT‑qPCR analysis revealed that Rg1 treatment significantly downregulated the transcriptional levels of Tlr4, Myd88, and Map3k7 in primary astrocytes, and reduced the expression and nuclear translocation of downstream NF‑κB pathway phosphoproteins (p‑IKKβ, p‑p65). Collectively, our data provide a molecular framework for the neuroprotective effects of Rg1 after SCI: Rg1 inhibits TLR4‑MyD88‑IKKβ/NF‑κB signaling, downregulates NLRP2 inflammasome‑mediated astrocyte pyroptosis, thereby alleviating neuroinflammation and promoting motor functional recovery. Importantly, the RNA‑seq analysis offers global molecular evidence identifying the key transcriptional pathway (TLR4/MyD88/NF‑κB) targeted by Rg1.

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Project description:Ginsenoside Rg1

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Large scale sequencing of PSTVd-RG1 sRNA in tomato plants

Project description:In order to analyze the production of small RNA (sRNA) by Potato spindle tuber viroid- RG1 strain (PSTVd-RG1) upon infecting the plants, the tomato plants (Lycopersicum esculentum cv. Rutgers) were inoculated with the PSTVd-RG1. After 21-days of post inoculation, total RNA was extracted and subjected for deep-sequencing using Illumina MiSeq platform. The primers were trimmed and the 21- to 24-nt long small RNA species were filtered after quality check of the raw data.

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Panax japonicus C. A. Myer LC-MSMS

Project description:Ginsenosides are a class of natural product triterpene saponins and almost exclusively in the plant genus Panax which has a long history of use as dietary supplements. Pharmacological research demonstrated that ginsenosides have multiple bioactivities. Ginsenoside is produced at high levels within Panax japonicus, and we have performed Lable-free quantitaion analysis of multiple tissues from this species in order to investigate the biosynthetic genes required for producing ginsenoside.

2023-02-09 | PXD038579 | Pride

Microbacterium sp. RG1

Project description:Microbacterium sp. RG1 Genome sequencing

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Chryseobacterium tagetis strain:RG1

Project description:Chryseobacterium tagetis strain:RG1 Genome sequencing and assembly

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Parallel analysis of 5'-RNA ends in PSTVd-RG1 inoculated tomato plants

Project description:5′ RNA ligase mediated rapid amplification of cDNA ends (5′ RLM-RACE) is the widely used tool for confirming the direct cleavage of target mRNA by a miRNA. Recently, 5′ RLM-RACE combined with high-throughput sequencing such as parallel analysis of RNA ends (PARE) are gaining importance as these techniques allows to assess the large number of sample in a single experiment. In order to validate the cleavage of predicted a target mRNA in potato spindle tuber viroid-RG1 (PSTVd-RG1) infected tomato plants, the leaf samples collected at 21-days post inoculation were subjected for RNA extraction. A custom RNA adaptor containing MmeI restriction endonuclease was ligated to the free 5'-phosphate end of an uncapped mRNA using T4 RNA ligase, followed by reverse transcription (RT) and second strand synthesis using poly (T) primers. Obtained product was slightly amplified, cleaved with MmeI restriction endonuclease and then, a dsDNA adaptor was added to the 3’-end of the MmeI restriction endonuclease cleavage site. Resulted products were sequence in an Illumina Mi-Seq machine. Retrieved data was then used to verify the 5’-termini of predicted target mRNA.

2017-09-20 | GSE70062 | GEO

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