Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Temporal expression profile of primary CD4+ T cell activation

ABSTRACT: Little is known about the global transcriptional program underlying CD4+ T-cell activation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of human CD4+ T-cell activation. The goal of this study was to identify genes involved in the various facets of human CD4+ T-cell activation. Keywords: time course

ORGANISM(S): Homo sapiens Thymus

PROVIDER: GSE7571 | GEO | 2007/12/29

SECONDARY ACCESSION(S): PRJNA100343

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human primary CD4+ T cell activation, time series

Project description:Little is known about the global transcriptional program underlying CD4+ T-cell activation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of human CD4+ T-cell activation. The goal of this study was to identify genes involved in the various facets of human CD4+ T-cell activation. Experiment Overall Design: CD4+ T cells isolated from peripheral blood were cultured with CD3, CD28, with or without IL-2 to induce T-cell activation. At each timepoint, cells were harvested and frozen for RNA isolation. Three biological replicate experiments were analyzed and approximately one-half of the samples from each experiment were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference thymus total RNA labeled with Cy5.

2008-06-15 | E-GEOD-7571 | biostudies-arrayexpress

Temporal expression profile of primary CD8+ T cell activation

2007-12-29 | GSE7572 | GEO

Temporal expression profile of primary T cell culture and T cell response to Oxidative stress

2007-12-29 | GSE6607 | GEO

Temporal expression profile of megakaryocytic differentiation primary CD34+ cell culture

Project description:Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of Mk differentiation human CD34-positive hematopoietic stem and progenitor cells cultured with thrombopoietin, interleukin-3, and Flt3-ligand. The goal this study was to identify genes involved in the various facets of Mk differentiation including commitment, polyploidization, proplatelet formation, and apoptosis. Keywords: time course

2006-11-08 | GSE3839 | GEO

Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 5% O2 levels

Project description:Little is known about the global transcriptional program underlying granulocytic (G) commitment, differentiation and maturation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of G differentiation of human CD34-positive hematopoietic stem and progenitor cells cultured with interleukin-3, interleukin-6 granulocyte colony stimulating factor and stem cell factor. The goal this study was to identify genes involved in the various facets of G differentiation including commitment, expansion, differentiation and functional capacity. Keywords: time course

2007-06-06 | GSE5918 | GEO

Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 20% O2 levels

2007-06-06 | GSE5917 | GEO

Temporal expression profile of primary CD4+ T cell activation

Project description:Temporal expression profile of primary CD4+ T cell activation

| PRJNA100343 | ENA

Temporal expression profile of granulocytic differentiation of primary CD34+ cells cultured under 5% and 20% O2 levels

2007-06-06 | GSE5922 | GEO

Transcription profiling of primary CD8+ T cell activation

Project description:Little is known about the global transcriptional program underlying CD8+ T-cell activation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of human CD8+ T-cell activation. The goal of this study was to identify genes involved in the various facets of human CD8+ T-cell activation. Experiment Overall Design: CD8+ T cells isolated from peripheral blood were cultured with CD3, CD28, with or without IL-2 to induce T-cell activation. At each timepoint, cells were harvested and frozen for RNA isolation. Three biological replicate experiments were analyzed and approximately one-half of the samples from each experiment were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference thymus total RNA labeled with Cy5.

2008-06-15 | E-GEOD-7572 | biostudies-arrayexpress

Transcription profiling of human primary T cell culture and T cell response to oxidative stress

Project description:Little is known about the global transcriptional program underlying T-cell activation and T-cell response to oxidative stress. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of human T-cell activation and T-cell response to H2O2 stress. The goal of this study was to identify genes involved in the various facets of human T-cell activation and T-cell response to H2O2 stress. Experiment Overall Design: T cells isolated from peripheral blood were cultured with CD3, CD28, and IL-2 to induce T-cell activaiton. Samples were splited into two parts before the cutlure started, one treated with 25 uM H2O2 for 10 minutes, the other intact. At each timepoint, cells were harvested and frozen for RNA isolation. Three biological replicate experiments were analyzed and approximately one-half of the samples from each experiment were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference thymus total RNA labeled with Cy5.

2008-06-15 | E-GEOD-6607 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data