Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HAL2 overexpression induces iron acquisition and enhanced salt resistance in bdf1Δ

ABSTRACT: The strain bdf1Δ+HAL2 improved salt resistance of bdf1∆. To gain further insight into the mechanism of bdf1∆ salt sensitivity, DNA microarray analysis was performed to determine the reason for the salt sensitivity of bdf1∆ cells and the process of how HAL2 overexpression and HDA1 deletion improves salt resistance. Transcriptomic analysis under salt treatment (0.5 mol.L-1 NaCl for 45 min) was performed using four different strains: bdf1∆, W303, bdf1Δ+HAL2 and bdf1∆hda1∆. The transcription of 1,393 genes were significantly changed( > 2-fold) in bdf1∆ compared with W303 upon NaCl stress (0.5 mol.L-1 NaCl for 45 min). Only 108 genes were significantly changed ( > 2-fold) in bdf1Δ+HAL2 compared with bdf1∆ upon NaCl stress (0.5 mol.L-1 NaCl for 45 min). Only 88 genes were significantly changed ( > 2-fold) in bdf1Δ+HAL2 compared with bdf1∆ upon NaCl stress (0.5 mol.L-1 NaCl for 45 min).

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE75828 | GEO | 2016/12/01

SECONDARY ACCESSION(S): PRJNA305490

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

The transcriptomic changes affected by BDF2 and SIR2 under salt stress

Project description:The strain bdf1∆bdf2∆[BDF2 L][SIR2 H] improved salt resistance of bdf1∆. To gain further insight into the mechanism of BDF1 in suppressing bdf1∆ salt sensitivity, DNA microarray analysis was performed to determine the reason for the salt sensitivity of bdf1∆ cells and the process of how coexpression of SIR2 and BDF2 improves salt resistance. Transcriptomic analysis under salt treatment (0.6 mol.L-1 NaCl for 45 min) was performed using three different strains: bdf1∆bdf2∆[BDF2 L][SIR2 H], bdf1∆bdf2∆[BDF2 L][pYX242] and bdf1∆[pRS316][pYX242]. The transcription of 3244 genes were significantly changed( > 2-fold) in bdf1∆bdf2∆[BDF2 L][SIR2 H] compared with bdf1∆[pRS316][pYX242] upon NaCl stress (0.6 mol.L-1 NaCl for 45 min). Only 281 genes were significantly changed ( > 2-fold) in bdf1∆bdf2∆[BDF2 L][pYX242] compared with bdf1∆[pRS316][pYX242] upon NaCl stress (0.6 mol∙L-1 NaCl for 45 min). BF2:bdf1∆[pRS316][pYX242]. BF2B: bdf1∆bdf2∆[BDF2 L][pYX242]. BF2S:bdf1∆bdf2∆[BDF2 L][SIR2 H].

2014-05-26 | GSE47367 | GEO

The transcriptomic changes affected by BDF2 and SIR2 under salt stress

Project description:The strain bdf1M-bM-^HM-^Fbdf2M-bM-^HM-^F[BDF2 L][SIR2 H] improved salt resistance of bdf1M-bM-^HM-^F. To gain further insight into the mechanism of BDF1 in suppressing bdf1M-bM-^HM-^F salt sensitivity, DNA microarray analysis was performed to determine the reason for the salt sensitivity of bdf1M-bM-^HM-^F cells and the process of how coexpression of SIR2 and BDF2 improves salt resistance. Transcriptomic analysis under salt treatment (0.6 mol.L-1 NaCl for 45 min) was performed using three different strains: bdf1M-bM-^HM-^Fbdf2M-bM-^HM-^F[BDF2 L][SIR2 H], bdf1M-bM-^HM-^Fbdf2M-bM-^HM-^F[BDF2 L][pYX242] and bdf1M-bM-^HM-^F[pRS316][pYX242]. The transcription of 3244 genes were significantly changed( > 2-fold) in bdf1M-bM-^HM-^Fbdf2M-bM-^HM-^F[BDF2 L][SIR2 H] compared with bdf1M-bM-^HM-^F[pRS316][pYX242] upon NaCl stress (0.6 mol.L-1 NaCl for 45 min). Only 281 genes were significantly changed ( > 2-fold) in bdf1M-bM-^HM-^Fbdf2M-bM-^HM-^F[BDF2 L][pYX242] compared with bdf1M-bM-^HM-^F[pRS316][pYX242] upon NaCl stress (0.6 molM-bM-^HM-^YL-1 NaCl for 45 min). BF2:bdf1M-bM-^HM-^F[pRS316][pYX242]. BF2B: bdf1M-bM-^HM-^Fbdf2M-bM-^HM-^F[BDF2 L][pYX242]. BF2S:bdf1M-bM-^HM-^Fbdf2M-bM-^HM-^F[BDF2 L][SIR2 H]. BF2B vs BF2 under salt treatment (0.6 mol.L-1 NaCl for 45 min); BF2S vs BF2 under salt treatment (0.6 mol.L-1 NaCl for 45 min).

2014-05-26 | E-GEOD-47367 | biostudies-arrayexpress

Rhodobacter sphaeroides salt stress response

Project description:Two excessive NaCl concentrations (0.25% and 1.5%) and two periods of treatment (7 min and 45 min) were used as moderate- and high-salt sress conditions at early and later stages of response, respectively. As a baseline, samples from cells grown on a standard SIS medium in the same growing system and same illumination were used. Keywords: time course and dose response

2007-06-22 | GSE8082 | GEO

Differential expression analysis of Thermus thermophilus HB8 wild-type strain in response to NaCl

Project description:In order to investigate response of T. thermophilus HB8 wild-type strain for high salt stress, we observed the expression profile of the total mRNA in the strain at 30 min after the addition of NaCl to a final concentration of 0.5% and/or 1.5%, and compared them with that of non-treated cells.

2010-10-15 | GSE21289 | GEO

Shewanella piezotolerans WP3 wild-type strain salt shock for 90 min

Project description:The WP3 strains was cultured at 20°C and salt shocked with 12% (w/v) NaCl for 90 min. The transcriptional profiles after and before salt shock were compared.

2017-01-01 | GSE82253 | GEO

Shewanella piezotolerans WP3 wild-type strain salt shock for 60 min

Project description:The WP3 strains was cultured at 20°C and salt shocked with 12% (w/v) NaCl for 60 min. The transcriptional profiles after and before salt shock were compared.

2017-01-01 | GSE82252 | GEO

Shewanella piezotolerans WP3 wild-type strain salt shock for 30 min

Project description:The WP3 strains was cultured at 20°C and salt shocked with 12% (w/v) NaCl for 30 min. The transcriptional profiles after and before salt shock were compared.

2017-01-01 | GSE82251 | GEO

RNA-seq analysis of gene expression in Helicobacter pylori expressing either WT Fur or a Fur variant (Fur-R88H).

Project description:Purpose: Next-generation sequencing (NGS) was used to analyze gene expression in H. pylori strains expressing either WT Fur or a Fur variant (i.e. Fur-R88H). The goals of this study are to examine the role that WT Fur and Fur-R88H play in affecting salt-responsive gene expression in the 2 different strains. The H. pylori strains were grown in the presence of routine salt (0.5% added NaCl) or high salt (1.0% added NaCl).

2023-01-06 | GSE214216 | GEO

Rhodobacter sphaeroides salt stress response

2010-06-10 | E-GEOD-8082 | biostudies-arrayexpress

della-regulation of salt stress responses-DELLAs contribute to salt stress responses

Project description:rs06-07_della - della-regulation of salt stress responses - Identification of DELLA-dependent dowtream targets in response to salt stress - Aim was to determine downstream target of DELLA proteins involved in salt stress tolerance. Wt, ga1-3, penta seeds were sterilized, sown on MS agar plates then put for stratification for 3 days at 4degreeC. Plates were placed in growth cabinet for 9 days. Seedlings were transferred to 24 well-plates, with 2 seedlings per well (0.5 ml MS liquid per well). Plates were placed in the same condition for 3 days. Finally, NaCl were added (final concentration 200 mM), except for the control. The salt treatment was applied for 30 min and 1h. Treatment was stopped by freezing in liquid nitrogen. Keywords: dose response,gene knock out,time course

2008-07-01 | GSE8556 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data