Transposon mutagenesis reveals fludarabine-resistance mechanisms in chronic lymphocytic leukemia

ABSTRACT: Purpose:To identify resistance mechanisms for the chemotherapeutic drug fludarabine in chronic lymphocytic leukemia (CLL), as innate and acquired resistance to fludarabine-based chemotherapy represents a major challenge for long-term disease control. Methods: We employed piggyBac transposon-mediated mutagenesis, combined with next-generation sequencing, to identify genes that confer resistance to fludarabine in a human CLL cell line. Results: RNA-seq profiling of fludarabine-resistant cells suggested deregulated MAPK signaling as involved in mediating drug resistance in CLL.

ORGANISM(S): Homo sapiens

PROVIDER: GSE75844 | GEO | 2016/03/31

SECONDARY ACCESSION(S): PRJNA305520

REPOSITORIES: GEO