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Gene expression profiles of the wild-type Cited4-transduced cells and the nontransduced cells.

ABSTRACT: We compared the gene expression profiles of the wild-type Cited4-transduced cells and the nontransduced cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE75887 | GEO | 2016/03/31

SECONDARY ACCESSION(S): PRJNA305620

REPOSITORIES: GEO

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Gene expression profiles of the wild-type Cited4-transduced cells and the nontransduced cells.

Project description:We compared the gene expression profiles of the wild-type Cited4-transduced cells and the nontransduced cells. Embryoid body was formed as in vitro cardiogenesis. For comparison of gene expression profiles of the wild-type Cited4-transduced cells and the nontransduced cells, total RNA was extracted from the wild-type Cited4-transduced cells at day 6.5 and the nontransduced cells at day 6.5.

2016-03-31 | E-GEOD-75887 | biostudies-arrayexpress

Gene expression profiles of the Cited4-siRNA-transduced cells and the non-transduced cells.

Project description:We compared the gene expression profiles of the Cited4-siRNA-transduced cells and the nontransduced cells.

2014-11-22 | GSE63542 | GEO

Gene expression profiles of the Cited4-siRNA-transduced cells and the non-transduced cells.

Project description:We compared the gene expression profiles of the Cited4-siRNA-transduced cells and the nontransduced cells. Embryoid body was formed as in vitro cardiogenesis. For comparison of gene expression profiles of the Cited4-siRNA-transduced cells and the nontransduced cells, total RNA was extracted from the Cited4-siRNA-transduced cells at day 6.5 and the nontransduced cells at day 6.5.

2014-11-22 | E-GEOD-63542 | biostudies-arrayexpress

Gene expression profiles of the Cited4 overexpressed and downregulated cells.

Project description:We compared the gene expression profiles of the Cited4 overexpressed and downregulated cells.

2016-03-31 | GSE63071 | GEO

Gene expression profiles of the Cited4 overexpressed and downregulated cells.

Project description:We compared the gene expression profiles of the Cited4 overexpressed and downregulated cells. Embryoid body was formed as in vitro cardiogenesis. For comparison of gene expression profiles of the Cited4 overexpressed and downregulated cells, total RNA was extracted from the Cited4 overexpressed and downregulated cells at day 6.5.

2016-03-31 | E-GEOD-63071 | biostudies-arrayexpress

Gene expression data from CITED4 knockout and control mouse hearts after transverse aortic constriction

Project description:Rationale: Cardiac CITED4 is induced by exercise and is sufficient to cause physiological hypertrophy and mitigate adverse ventricular remodeling after ischemic injury. However, the role of endogenous CITED4 in response to physiological or pathological stress is unknown. Objective: To investigate the role of CITED4 in murine models of exercise and pressure overload. Methods and Results: We generated cardiomyocyte-specific CITED4 knockout mice (C4KO) and subjected them to an intensive swim exercise protocol as well as transverse aortic constriction (TAC). Echocardiography, western blotting, qPCR, immunohistochemistry, immunofluorescence, and transcriptional profiling for mRNA and miRNA expression were performed. Cellular crosstalk was investigated in vitro. CITED4 deletion in cardiomyocytes did not affect baseline cardiac size or function in young adult mice. C4KO mice developed modest cardiac dysfunction and dilation in response to exercise. After TAC, C4KOs developed severe heart failure with left ventricular dilation, impaired cardiomyocyte growth accompanied by reduced mammalian target of rapamycin (mTOR) activity and maladaptive cardiac remodeling with increased apoptosis, autophagy, and impaired mitochondrial signaling. Interstitial fibrosis was markedly increased in C4KO hearts after TAC. RNAseq revealed induction of a pro-fibrotic miRNA network. miR30d was decreased in C4KO hearts after TAC and mediated crosstalk between cardiomyocytes and fibroblasts to modulate fibrosis. miR30d inhibition was sufficient to increase cardiac dysfunction and fibrosis after TAC. Conclusions: CITED4 protects against pathological cardiac remodeling by regulating mTOR activity and a network of miRNAs mediating cardiomyocyte to fibroblast crosstalk. Our findings highlight the importance of CITED4 in response to both physiological and pathological stimuli.

2020-04-30 | GSE149584 | GEO

Transcriptional response to Rosiglitazone in adipocyte progenitors isolated from WT and Cited4-KO scWAT

Project description:Lin-Sca-1+ adipocyte progenitors from subcutaneous adipose tissue of wild-type and Cited4-/- female mice were cultured under conditions promoting general adipogenic differentiation or beige/brite adipocyte differentiation (treatment with Rosi). Cells were harvested 48 post-induction of differentiation.

2018-07-04 | E-MTAB-6796 | biostudies-arrayexpress

miRNA expression data from CITED4 knockout and control mouse hearts after transverse aortic constriction

Project description:To investigate the role of CITED4 in a murine model of cardiac pressure overload

2020-05-12 | GSE150293 | GEO

Analysis of CITED4 overexpression in the CRC cell line SW480

Project description:Ires-GFP and CITED4-ires-GFP overexpressing permanent cell lines were generated by transfection of the CRC cell line SW480 with either the ires-GFP containing vector vector (in pCDNA4HISMAX) or the CITED4-ires GFP in (pCDNA4HISMAX) and selected with 2 ug/ ml puromycin in order to prepare permanent cell lines. For microarray analysis, the two cell lines were plated at a density of 1 x 10E5 cell in 12 well plates in 1 ml RPMI plus 10% FCS and penicillin/ streptomycin. The respective cell lines were harvested at day 1, 3 and 5 for RNA preparation and microarray analysis using Agilent human 4 x 44k expression microarrays. Every time point (cy3 labeled) was hybridzed together with Stratagene Human Total RNA standard as a control (cy5). Comparisons were made between the CITED4 overexpressing permanent cell line and ires-GFP containing cell line.

2014-11-01 | E-MTAB-2486 | biostudies-arrayexpress

Analysis of CITED4 shRNA mediated knockdown in the CRC cell line SW480

Project description:Empty vector, control shRNA and CITED4 shRNA permanent cell lines were generated by lentiviral transduction of the CRC cell line SW480 with either the empty vector (plko1), a control shRNA containing vector or a CITED4 shRNA containing vector and selected with puromycin in order to prepare permanent cell lines. For microarray analysis, the three cell lines were plated at a density of 1 x 10E5 cell in 12 well plates in 1 ml RPMI plus 10% FCS and penicillin/ streptomycin. The respective cell lines were harvested at day 1, 3 and 5 for RNA preparation and microarray analysis using Agilent human 4 x 44k expression microarrays. Every time point (cy3 labeled) was hybridzed together with Stratagene Human Total RNA standard as a control (cy5). Comparisons were made between the CITED4 shRNA permanent cell line and either the control shRNA or empty vector cell line.

2014-11-01 | E-MTAB-2487 | biostudies-arrayexpress

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