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H3K27me3 histone modification in A549 cells

ABSTRACT: Examination of H3K27me3 histone modification in A549 cell line

ORGANISM(S): Homo sapiens

PROVIDER: GSE75903 | GEO | 2015/12/11

SECONDARY ACCESSION(S): PRJNA305653

REPOSITORIES: GEO

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H3K27me3 histone modification in A549 cells

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Examination of H3K27me3 histone modification in 3 cell types in presence or absence of SuUR mutation

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Perturbation of the relative abundance of H2A.Z variants alters H3K27me3 enrichment in Arabidopsis thaliana - Untargeted Analysis

Project description:H3K27me3 is a histone modification associated with transcriptional repression that plays a pivotal role in differentiation and development in plants and animals. H2A.Z, a histone variant of H2A, is often colocalized with H3K27me3 in Arabidopsis. Previous genome-wide studies have raised the possibility that H2A.Z contributes to H3K27me3 homeostasis in plants. To examine the contribution of H2A.Z to H3K27me3, we utilized hta9 hta11, a plant line which is defective in two of three genes that code for H2A.Z and has been previously characterized as an H2A.Z-depletion mutant. Surprisingly, ChIP-seq analysis of H2A.Z enrichment revealed that hta9 hta11 plants exhibit a nearly indistinguishable genic H2A.Z enrichment pattern relative to wild type with few genes that are enriched or depleted for H2A.Z. Mass spectrometry measurement of histone protein abundance in hta9 hta11 plants in conjunction with our ChIP-seq analysis indicates that the chromatin of hta9 hta11 plants exhibits altered relative abundance of H2A.Z variants rather than a global depletion of H2A.Z. In contrast, ChIP-seq analysis of H3K27me3 reveals that hta9 hta11 plants exhibit a robust global reduction in H3K27me3 enrichment, and numerous genes are identified that exhibit altered levels for H3K27me3. In addition, hta9 hta11 plants exhibit differential expression of thermoresponsive genes that have previously been shown to be dependent on H2A.Z. Our combined analyses indicate that specific H2A.Z isoforms play distinct roles in chromatin composition and gene expression in Arabidopsis, particularly with regards to H3K27me3 homeostasis.

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