Transcriptomics,Genomics

Dataset Information

24

Microarray of SIRT6 KO in hepatocellular carcinoma


ABSTRACT: We identified the specific role of Sirtuin 6 (SIRT6) in the progression of hepatocellular carcinoma (HCC) and evaluated its therapeutic and prognostic potential. SIRT6 knockdown by shRNA inhibited the proliferation of HCC cells; moreover, depletion of SIRT6 suppressed HCC tumor growth in vivo. SIRT6 silencing significantly down regulated the growth of HCC cell lines via induction of cellular senescence, which was attributed by DNA damage in p16/Rb and p53/p21-independent manners. We also showed that SIRT6 knockdown increased the number of G2/M phase arrested cells by increasing cyclin B1 and 14-3-3-∂. Moreover, SIRT6 levels were significantly higher in HCC cell lines than a normal hepatic cell line, and were higher in HCC than non-tumor tissues. Aberrant SIRT6 expression correlated with poor prognostic features of HCC. Taken together, our findings suggest SIRT6 may act as a tumor promoter by preventing cellular senescence attributed by DNA damage and suggests that targeting SIRT6 could be a promising therapeutic approach for cancer treatment. Overall design: Two-condition experiment, Control vs. SIRT6 KO. Biological replicates: 2 control replicates, 2 KO replicates.

INSTRUMENT(S): Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)

SUBMITTER: Dae-Kyum Kim 

PROVIDER: GSE75905 | GEO | 2017-02-09

SECONDARY ACCESSION(S): PRJNA305655

REPOSITORIES: GEO

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Publications

SIRT6 Depletion Suppresses Tumor Growth by Promoting Cellular Senescence Induced by DNA Damage in HCC.

Lee Namgyu N   Ryu Hye Guk HG   Kwon Jung-Hee JH   Kim Dae-Kyum DK   Kim Sae Rom SR   Wang Hee Jung HJ   Kim Kyong-Tai KT   Choi Kwan Yong KY  

PloS one 20161108 11


The role of Sirtuin 6 (SIRT6) as a tumor suppressor or oncogene in liver cancer remains controversial. Thus, we identified the specific role of SIRT6 in the progression of hepatocellular carcinoma (HCC). SIRT6 expression was significantly higher in HCC cell lines and HCC tissues from 138 patients than in an immortalized hepatocyte cell line, THLE-2 and non-tumor tissues, respectively. SIRT6 knockdown by shRNA suppressed the growth of HCC cells and inhibited HCC tumor growth in vivo. In addition,  ...[more]

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