Control, low water potential (drought) stress and stress release transcriptome analysis of mutants impaired in proline (Delta1-pyrroline-5-carboxylate synthase1-4; p5cs1-4) or abscisic acid (aba2-1) synthesis.

ABSTRACT: mRNA sequencing was used to identify genes differentially expressed in Delta1-pyrroline-5-carboxylate synthase1-4 (p5cs1-4) mutants deficient in low water potential-induced proline accumulation and the Abscisic Acid (ABA) deficient mutant aba2-1 compared to Col-0 wild type. Three treatments were analyzed for each genotype: unstressed control, a 96 hour low water potential (-1.2 MPa) treatment and a stress-release treatment where seedlings were removed from the stress treatment and transferred back to control media for 10 hours. Approximately 100 genes were found to be significantly up-regulated in p5cs1-4 compared to wild type in both the control and stress treatments with an equal number down regulated. There was also a substantial transcription response to the stress release treatment which varied between wild type, p5cs1-4 and aba2-1.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE75933 | GEO | 2016/08/05

SECONDARY ACCESSION(S): PRJNA305733

REPOSITORIES: GEO