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Development of gene expression signature for primary epidermal neonatal keratinocytes treated with mature IL-36B cytokine

ABSTRACT: Stimulated primary keratinocytes with mature IL-36B cytokine and analysed differential mRNA expression at 8 h timepoint.

ORGANISM(S): Homo sapiens

PROVIDER: GSE75967 | GEO | 2015/12/15

SECONDARY ACCESSION(S): PRJNA305869

REPOSITORIES: GEO

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Development of gene expression signature for primary epidermal neonatal keratinocytes treated with mature IL-36B cytokine

Project description:Stimulated primary keratinocytes with mature IL-36B cytokine and analysed differential mRNA expression at 8 h timepoint IL-36B induced gene expression in primary human neonatal keratinocytes was measured at 8 hours after exposure to dose IL-36B (5 nM).

2015-12-15 | E-GEOD-75967 | biostudies-arrayexpress

Cytokine treated normal human epidermal keratinocytes

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Human cathelicidin antimicrobial peptide LL37 effect on human neonatal epidermal keratinocytes (NHEKs).

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Human cathelicidin antimicrobial peptide LL37 effect on human neonatal epidermal keratinocytes (NHEKs).

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Gene expression profile of human epidermal keratinocytes in response to IFNgamma, IL-4, IL-6, IL-17A and IL-22

Project description:To understand the role of epidermal keratinocytes in immunopathology of skin diseases with predominant T helper (Th) cell responses, we measured the genome-wide transcriptional profile of human keratinocytes in response to IFNgamma, IL-4, IL-17A or IL-22, major cytokines produced by Th1, Th2, Th17 or Th22 cells, respectively. IL-6 was also included in the transcriptional profile analysis because a variety of pro-inflammatory stimuli stimulate human keratinocytes to produce IL-6 that has an autocrine or paracrine role in epidermal immunity. We aimed to discover commonly expressed genes in human keratinocytes in response to pro-inflammatory cytokines, which would be associated with common pathophysiological responses in various skin diseases such as skin permeability barrier disruption or epidermal hyperplasia. Normal human keratinocytes (NHKs) were stimulated with IFNγ, IL-4, IL-6, IL-17A and IL-22 for 24 hours and harvested for total RNA extraction and hybridization on Affymetrix microarrays.

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Transcription profiling time series of human epidermal keratinocytes treated with Oncostatin-M

Project description:Cultured epidermal keratinocytes treated with OsM 1, 4, 24 & 48hrs, and Skinethic epidermal substitutes treated 1, 4, 24, 48h & 7days, each with untreated control Experiment Overall Design: Each timepoint has treated/untreated matched control. Experiment Overall Design: Skinethic 4h treated was replicated. Experiment Overall Design: Note: OncostatinM treatment, GSM61488, is the 1h control!!

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Gene expression profile of human epidermal keratinocytes in response to IFNgamma, IL-4, IL-6, IL-17A and IL-22

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2014-11-04 | E-GEOD-62939 | biostudies-arrayexpress

Transcription profiling of human epidermal keratinocytes treated with IL-1

Project description:Interleukin-1 is a proinflammatory and immunomodulatory cytokine that plays a crucial role in inflammatory diseases of the skin, including bacterial infections, bullous diseases, UV damage and especially psoriasis. To characterize the molecular effects of IL-1 in epidermis, we defined the transcriptional changes in human epidermal keratinocytes 1, 4, 24, and 48 h after treatment with IL-1a. IL-1 significantly regulated 388 genes, including genes associated with proteolysis, adhesion, signal transduction, proliferation, and epidermal differentiation. IL-1 induces many genes that have antimicrobial function. Secreted cytokines, chemokines, growth factors, and their receptors are the prominent targets of IL-1 regulation, including IL-8, IL-19, elafin, C3, and S100A proteins, which implicates IL-1 in the pathogenesis of inflammatory diseases. IL-1 induced not only proliferation-associated genes but also differentiation marker genes such as transglutaminase-1 and involucrin, which suggests that IL-1 plays an important role in the aberrant proliferation and differentiation seen in psoriasis. Correlation of IL-1 regulated genes with the TNFa and IFNg regulated ones showed more similarities between IL-1 and TNFa than IL-1 and IFNg, whereas Oncostatin-M affected a largely unrelated set of genes. IL-1 regulates many genes previously shown to be specifically over-expressed in psoriasis. In summary, IL-1 regulates a characteristic set of genes that define its specific contribution to inflammation and aberrant differentiation in skin diseases. Experiment Overall Design: keratinocytes were treated with 25 ng/ml IL-1 and transcriptomes compared to untreated controls at 1, 4, 24 and 48 h post treatment.

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Transcriptomic Analysis of Human Epidermal Keratinocytes Stimulated with TWEAK, TNF and IL-17A

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