Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ZCF32, a fungus specific Zn(II)2-Cys6 transcription factor, is a major regulator of the biofilm circuit in the human pathogen Candida albicans

ABSTRACT: To delineate the functional role of ZCF32 in Candida albicans biology, we carried out the genome wide expression analysis of wild-type and zcf32 null mutants by global expression array. We analysed the gene expression profiles of YPK102/1 and YPK102/2 and the parent wild type (SC5314) strain grown in YPD liquid medium at 30°C till the start of the stationary phase. In the microarray analysis, a total of 607 genes were found to be differentially expressed (fold change < 1.5 at p-value < 0.05) between the wild type and two zcf32 null mutants. Out of the 607 differentially expressed genes, 438 genes were up-regulated whereas 169 genes were down-regulated. Gene ontology (GO) analysis of up-regulated and down-regulated genes suggests that biofilm, filamentation, iron homeostasis, cell wall biogenesis, oxidation, and cell cycle are the major pathways altered in zcf32 null mutant.

ORGANISM(S): Candida albicans

PROVIDER: GSE76165 | GEO | 2016/12/31

SECONDARY ACCESSION(S): PRJNA306506

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Regulation of the biofilm-specific genes circuitry in Candida albicans by a novel histone H3 variant

Project description:To understand the biological relevance of the role played by the HHT1 histone H3 variant in C. albicans, we performed a transcriptome analysis of the mutant by global gene expression array analysis. We analyzed gene expression profile of the mutant in strains LR107 and LR108 (hht1∆/hht1∆) and the parent wild type (SC5314) strain grown in YPD liquid medium at 30°C. In the microarray analysis, a total of 1222 genes were found to be expressed significantly different (fold change < 1.5 at p-value < 0.05) between wild type and two hht1 null mutants. Out of the 1222 differentially expressed genes, 670 genes were up-regulated whereas 552 genes were down-regulated. Gene ontology (GO) analysis of up-regulated and down-regulated genes suggests that biofilm, Hap43, transcription, filamentation, white-opaque/mating and invasive growth are the major pathways altered in minor H3 null mutant. Transcription profiling in planktonic YPD condition suggests that minor H3 represses biofilm genes in the planktonic mode of growth. Therefore we performed a genome-wide expression array study after growing the wild type strain and hht1/hht1 mutant strains in Spider induced biofilm condition. This experiment was carried out with two biological replicates of hht1 null mutants. The biofilm induced microarray data revealed that a total of 1010 genes were differentially expressed (at p-value <0.05 and fold change <1.5) between the wild type and hht1 mutants. Out of 1016 differentially expressed genes, 572 genes were up-regulated whereas 444 genes were down-regulated.

2016-09-08 | GSE72824 | GEO

Regulation of the biofilm-specific genes circuitry in Candida albicans by a novel histone H3 variant

Project description:To understand the biological relevance of the role played by the HHT1 histone H3 variant in C. albicans, we performed a transcriptome analysis of the mutant by global gene expression array analysis. We analyzed gene expression profile of the mutant in strains LR107 and LR108 (hht1â/hht1â) and the parent wild type (SC5314) strain grown in YPD liquid medium at 30Â°C. In the microarray analysis, a total of 1222 genes were found to be expressed significantly different (fold change < 1.5 at p-value < 0.05) between wild type and two hht1 null mutants. Out of the 1222 differentially expressed genes, 670 genes were up-regulated whereas 552 genes were down-regulated. Gene ontology (GO) analysis of up-regulated and down-regulated genes suggests that biofilm, Hap43, transcription, filamentation, white-opaque/mating and invasive growth are the major pathways altered in minor H3 null mutant. Transcription profiling in planktonic YPD condition suggests that minor H3 represses biofilm genes in the planktonic mode of growth. Therefore we performed a genome-wide expression array study after growing the wild type strain and hht1/hht1 mutant strains in Spider induced biofilm condition. This experiment was carried out with two biological replicates of hht1 null mutants. The biofilm induced microarray data revealed that a total of 1010 genes were differentially expressed (at p-value <0.05 and fold change <1.5) between the wild type and hht1 mutants. Out of 1016 differentially expressed genes, 572 genes were up-regulated whereas 444 genes were down-regulated. Agilent custom one-color experiment,Organism:Candida albicans, Whole Genome Candida albicans 8x15K (AMADID: 026377) designed by Genotypic Technology Private Limited.

2016-09-08 | E-GEOD-72824 | biostudies-arrayexpress

E-MEXP-3867

Project description:Transcription profiling by array of A. thaliana U11U12-65k mutant vs Col-0 wild type to investigate differently regulated genes

2015-06-05 | E-MEXP-3867 | ExpressionAtlas

E-GEOD-8188

Project description:Transcription profiling of maize zmet2-m1 mutants relative to wild-type

2020-04-14 | E-GEOD-8188 | ExpressionAtlas

E-GEOD-19257

Project description:Transcriptome differences between two vitamin C-deficient mutants and wild type

2015-12-02 | E-GEOD-19257 | ExpressionAtlas

Transcriptomic and phenotypic studies of Listeria monocytogenes NmlR in stress response

Project description:The goal of this study was to compare the transcriptome between wild type strain of Listeria monocytogenes and delete nmlR mutant strain of L. monocytogenes using NGS. Method: Duplicate samples of rRNA depleted RNA from wild type and mutants were used to study transcriptomes by ion torrent platform. Transcriptomes of wild type and nmlR mutant were compared by EDGE-pro program. Result: Differential expression by EDGE-pro showed 74 genes with differential expressions between wild type and nmlR null mutant (46 genes were negatively regluated and 28 genes were positively regulated by NmlR).

2016-07-01 | GSE70265 | GEO

Expression data from ozone-treated wild-type and G-protein null mutant Arabidopsis lines

Project description:Ozone pollution decreases plant growth and yield worldwide. Some of the effects are genetically-mediated and are reported to involve G-protein signaling pathways. Effects of ozone on gene expression were examined in wild-type and G-protein null mutants to determine affected genes and to determine differential responses that may help define affected pathways. We used microarrays to examine changes in gene expression in response to ozone exposure and identified distinct classes of up- and down-regulated genes in wild-type and G-protein null mutant genotypes.

2011-12-24 | GSE34667 | GEO

Transcriptomes of C. albicans aox2 deletion mutant or wild-type C. albicans SC5314 treated with pyocyanin

Project description:We compared the early gene expression profiles (30 min) of log-phase wild type C. albicans SC5314 growing in YPD at 30 °C to an aox2 deletion mutant or to wild-type SC5314 in response to 80 micromolar pyocyanin or 0.5 mM SHAM

2019-07-26 | GSE117717 | GEO

Whole transcriptomic sequencing of zebrafish rx3 mutants during optic vesicle morphogenesis

Project description:To identify genes regulated by Rx3 during optic vesicle morphogenesis, adult zebrafish carriers of a null rx3 mutation were mated. Before 13 hours post fertilization (hpf), the earliest time point at which optic vesicle evagination phenotypes could be reliably detected, offspring were phenotypically separated into pools comprising of mutants with an absence of optic vesicles or siblings exhibiting a wild-type phenotype. Three replicates of pooled RNA samples from 13 hpf eyeless mutants (rx3-/-) or phenotypically wild-type siblings (rx3+/+ or rx3+/-), and one replicate of 13 hpf wild-type zebrafish larva were collected for whole transcriptome sequencing.

2014-09-30 | GSE52652 | GEO

E-GEOD-39214

Project description:RNA-seq of coding RNA of Arabidopsis Phytochrome (phy)-Interacting bHLH Factor (PIF) mutants against wild-type controls to study target genes of PIF

2019-05-14 | E-GEOD-39214 | ExpressionAtlas

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data