Dataset Information


Sp7/Osterix is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification [RNA-Seq]

ABSTRACT: Sp7/Osterix is a master regulator of osteoblast specification. To identify transcripts profile in Sp7 positive osteoblast, we performed RNA-seq on primary mouse calvarial cells obtained from Sp7-GFP reporter mice at P1. By hierarchical clustering using transcriptional profiles for chondrocytes and mouse embryonic fibroblasts (MEFs) together with the osteoblast data, we identified cell-type enriched gene expression signatures in osteoblasts, chondrocytes and MEFs. In conjunction with Sp7 ChIP-seq in osteoblast, we identified putative Sp7 targets which underlie the osteoblast regulatory program. Overall design: RNA-seq experiments with GFP-sorted Sp7 positive osteoblasts and chondrocytes isolated from wild-type rib cartilage at P1

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Hironori Hojo  

PROVIDER: GSE76186 | GEO | 2016-04-28



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Sp7/Osterix Is Restricted to Bone-Forming Vertebrates where It Acts as a Dlx Co-factor in Osteoblast Specification.

Hojo Hironori H   Ohba Shinsuke S   He Xinjun X   Lai Lick Pui LP   McMahon Andrew P AP  

Developmental cell 20160428 3

In extant species, bone formation is restricted to vertebrate species. Sp7/Osterix is a key transcriptional determinant of bone-secreting osteoblasts. We performed Sp7 chromatin immunoprecipitation sequencing analysis identifying a large set of predicted osteoblast enhancers and validated a subset of these in cell culture and transgenic mouse assays. Sp family members bind GC-rich target sequences through their zinc finger domain. Several lines of evidence suggest that Sp7 acts differently, enga  ...[more]

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