Dataset Information


Pax6 links H3K4 methylase activity to transcriptional regulation of Plekha1 through its distal enhancer

ABSTRACT: We performed ChIP-seq on mouse lens epithelial cells (αTN4) and mouse newborn lens. Genome-wide binding sites of Pax6, H3K4me1, H3K4me2, H3K4me3, and RNA polymerase II were generated. We also performed RNA-seq on αTN4 cells treated with Pax6 and control shRNAs. Overall design: Examination of correlation between Pax6 binding and histone H3K4 methylation distribution.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Ales Cvekl  

PROVIDER: GSE76315 | GEO | 2017-02-10



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Pax6 associates with H3K4-specific histone methyltransferases Mll1, Mll2, and Set1a and regulates H3K4 methylation at promoters and enhancers.

Sun Jian J   Zhao Yilin Y   McGreal Rebecca R   Cohen-Tayar Yamit Y   Rockowitz Shira S   Wilczek Carola C   Ashery-Padan Ruth R   Shechter David D   Zheng Deyou D   Cvekl Ales A  

Epigenetics & chromatin 20160909 1

Pax6 is a key regulator of the entire cascade of ocular lens formation through specific binding to promoters and enhancers of batteries of target genes. The promoters and enhancers communicate with each other through DNA looping mediated by multiple protein-DNA and protein-protein interactions and are marked by specific combinations of histone posttranslational modifications (PTMs). Enhancers are distinguished from bulk chromatin by specific modifications of core histone H3, including H3K4me1 an  ...[more]

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