Transcriptomics,Genomics

Dataset Information

240

Single Cell RNA-seq Study of Midbrain and Dopaminergic Neuron Development in Mouse, Human, and Stem Cells


ABSTRACT: In order to get a better molecular understanding of human midbrain development, this study defines cell types of the ventral midbrain in both human and mouse as well as stem cell-derived dopaminergic neuron preparations, and reveals the temporal dynamics of key lineages across development and the adult. Overall design: Single cells according to Samples list consisting of: Human embryo ventral midbrain cells between 6 and 11 weeks of gestation, mouse ventral midbrain cells at six developmental stages between E11.5 to E18.5, Th+ neurons at P19-P27, and putative dopaminergic neurons at P28-P56 FACS-sorted from Slc6a3-Cre/tdTomato mice. Stem cells (iPSCs and ESCs) preparation at different stages of differentiation towards Th+ neurons.

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

SUBMITTER: Sten Linnarsson 

PROVIDER: GSE76381 | GEO | 2016-10-06

SECONDARY ACCESSION(S): PRJNA307121

REPOSITORIES: GEO

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Publications


Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA sequencing to examine ventral midbrain development in human and mouse. We found 25 molecularly defined human cell types, including five subtypes of radial glia-like cells and four progenitors. In the mouse, two mature fetal dopaminergic neuron s  ...[more]

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