CLKGR in S2 cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE7644 | GEO | 2007/07/09
SECONDARY ACCESSION(S): PRJNA105549
REPOSITORIES: GEO
Similar Datasets
Project description:Experiments performed in S2 cells to identify direct CLK targets Experiment Overall Design: Those targets were determined using Drosophilagenome1 and Drosophila 2.0 Affymetrix CHIPS.
2008-06-15 | E-GEOD-7644 | biostudies-arrayexpress
Project description:S2 cells transfected with pAc-Clk or empty vector Keywords: S2 cells Clk
2007-07-09 | GSE7653 | GEO
Project description:S2 cells transfected with pAc-Clk or empty vector Experiment Overall Design: S2 cells transfected with pAc-Clk or empty vector. Cells were transfected and RNA collected after 48hs
2008-06-15 | E-GEOD-7653 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series:; GSE7644: CLKGR in S2 cells; GSE7646: CLK targets from fly heads; GSE7651: Timepoints 5073 strain; GSE7652: Timepoints Control strain; GSE7653: S2 cells transfected with Clk Experiment Overall Design: Refer to individual Series
2008-06-16 | E-GEOD-8391 | biostudies-arrayexpress
Project description:modENCODE_submission_2753 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known replication initiation complexes. These experiments will be conducted in multiple cell types and developmental tissues. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-seq EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; EXPERIMENTAL FACTORS: Antibody dORC2
2010-03-16 | E-GEOD-20887 | biostudies-arrayexpress
Project description:Timecourse of 9-(S)-HODE treatment of Drosophila S2 cells. 9-(S)-HODE is an oxygenated metabolite of the abundant, 18 carbon, omega-6 PUFA linoleic acid. To discriminate how omega-6 PUFAs affect meta-inflammation S2 cells were with 10uM for 12 and 24hrs and microarrays performed to identify downstream transcriptional targets
2018-08-12 | E-MTAB-6253 | biostudies-arrayexpress
Project description:CLOCK (CLK) is a master transcriptional regulator of the circadian clock in Drosophila. To identify CLK direct target genes and address circadian transcriptional regulation in Drosophila, we performed chromatin immunoprecipitation-tiling array assays (ChIP-chip) with a number of circadian proteins. CLK binding cycles on at least 800 sites with maximal binding in the early night. The CLK partner protein CYCLE (CYC) is on most of these sites. The CLK/CYC heterodimer is joined 4-6 hrs later by the transcriptional repressor PER, indicating that the majority of CLK targets are regulated similarly to core circadian genes (Menet et al. 2010). About 30% of target genes also show cycling Pol II binding. Many of these generate cycling RNAs despite not being documented in prior RNA cycling studies. This is due in part to different RNA isoforms and to fly head tissue heterogeneity. CLK has specific targets in different tissues, implying that important CLK partner proteins and/or mechanisms contribute to gene-specific and tissue-specific regulation.
2011-11-15 | GSE32613 | GEO
Project description:Little is known about the contribution of translational control to circadian rhythms. To address this issue and in particular translational control by microRNAs (miRNAs), we knocked down the miRNA biogenesis pathway in Drosophila circadian tissues. In combination with an increase in circadian-mediated transcription, this severely affected Drosophila behavioral rhythms, indicating that miRNAs function in circadian timekeeping. To identify miRNA–mRNA pairs important for this regulation, immunoprecipitation of AGO1 followed by microarray analysis identified mRNAs under miRNA-mediated control. They included three core clock mRNAs—clock (clk), vrille (vri), and clockworkorange (cwo). To identify miRNAs involved in circadian timekeeping, we exploited circadian cell-specific inhibition of the miRNA biogenesis pathway followed by tiling array analysis. This approach identified miRNAs expressed in fly head circadian tissue. Behavioral and molecular experiments show that one of these miRNAs, the developmental regulator bantam, has a role in the core circadian pacemaker. S2 cell biochemical experiments indicate that bantam regulates the translation of clk through an association with three target sites located within the clk 39 untranslated region (UTR). Moreover, clk transgenes harboring mutated bantam sites in their 39 UTRs rescue rhythms of clk mutant flies much less well than wild-type CLK transgenes.
2009-08-31 | GSE17627 | GEO
Project description:CLK targets from fly heads using the TIM-GAL4; UAS-CLKGR line Keywords: CLK TARGETS
2007-07-09 | GSE7646 | GEO
Project description:Little is known about the contribution of translational control to circadian rhythms. To address this issue and in particular translational control by microRNAs (miRNAs), we knocked down the miRNA biogenesis pathway in Drosophila circadian tissues. In combination with an increase in circadian-mediated transcription, this severely affected Drosophila behavioral rhythms, indicating that miRNAs function in circadian timekeeping. To identify miRNA–mRNA pairs important for this regulation, immunoprecipitation of AGO1 followed by microarray analysis identified mRNAs under miRNA-mediated control. They included three core clock mRNAs—clock (clk), vrille (vri), and clockworkorange (cwo). To identify miRNAs involved in circadian timekeeping, we exploited circadian cell-specific inhibition of the miRNA biogenesis pathway followed by tiling array analysis. This approach identified miRNAs expressed in fly head circadian tissue. Behavioral and molecular experiments show that one of these miRNAs, the developmental regulator bantam, has a role in the core circadian pacemaker. S2 cell biochemical experiments indicate that bantam regulates the translation of clk through an association with three target sites located within the clk 39 untranslated region (UTR). Moreover, clk transgenes harboring mutated bantam sites in their 39 UTRs rescue rhythms of clk mutant flies much less well than wild-type CLK transgenes. Tilling arrays were hybridized with cDNA from control or flies in which Drosha or Pasha was downregulated in the timeless-expressing neurons.
2009-08-31 | GSE17628 | GEO