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SOX9 Drives WNT Pathway Activation in Prostate Cancer [ChIP-seq]

ABSTRACT: SOX9 is critical for prostate development and is implicated in prostate cancer, we used SOX9 ChIP-seq in combination with transcriptome profiling to identify genes and pathways it regulates in normal or neoplastic epithelium.

ORGANISM(S): Homo sapiens

PROVIDER: GSE76451 | GEO | 2016/12/28

SECONDARY ACCESSION(S): PRJNA307344

REPOSITORIES: GEO

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SOX9 Drives WNT Pathway Activation in Prostate Cancer [gene expression]

Project description:SOX9 is critical for prostate development and is implicated in prostate cancer, we used transcriptome profiling in combination with SOX9 ChIP-seq to identify genes and pathways it regulates in normal or neoplastic epithelium. We used microarrays to detail the global programme of gene expression in TMPRSS2/ERG fusion positive prostate cancer cell line with high basal expression of SOX9 by comparing the expression changes between SOX9 knockdown versus control.

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SOX9 Drives WNT Pathway Activation in Prostate Cancer

Project description:SOX9 Drives WNT Pathway Activation in Prostate Cancer

| PRJNA307342 | ENA

SOX9 drives WNT pathway activation in prostate cancer.

Project description: Not available

| S-EPMC4855922 | biostudies-literature

SOX9 Drives WNT Pathway Activation in Prostate Cancer

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-12-28 | GSE76452 | GEO

Sox9 mediated differential gene expression was assessed in prostaspheres derived from urogenital sinus epithelial cells

Project description:Prostate cancer is one of the most common malignancies and the second leading cause of death from cancer in men. The molecular mechanisms driving prostate carcinogenesis are complex; with several lines of evidence suggesting that the re-expression of conserved developmental programs play a key role. Through conditional gene targeting and organ grafting, we describe conserved roles for the transcription factor Sox9 in the initiation of both prostate organogenesis and prostate carcinogenesis in murine models. Abrogation of Sox9 expression prior to the initiation of androgen signaling blocks the initiation of prostate development. Similarly, Sox9 deletion in two genetic models of prostate cancer (TRAMP and Hi-Myc) blocks cancer initiation. Expression profiling of Sox9-null prostate epithelial cells reveals that the role of Sox9 in the initiation of prostate development may relate to its regulation of multiple cytokeratins and/or calcium-related proteins. Due to its essential role in cancer initiation, manipulation of Sox9 targets in at-risk men may prove useful in the chemoprevention of prostate cancer. Sox9 differential gene expression in prostaspheres derived from the urogenital sinus epithelium was assessed by tow-colors direct comparisons of labeled moieties. Hybridizations were performed on the Agilent (Santa Clara, CA) Whole Mouse Genome DNA microarray (mgug4122a). Sox9 targets were assessed in murine prostate epithelial cells with targeted deletion.

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Prostate CancerM-bM-^@M-^SAssociated Gene Expression Alterations Determined from Needle Biopsies

Project description:To accurately identify gene expression alterations that differentiate neoplastic from normal prostate epithelium using an approach that avoids contamination by unwanted cellular components and is not compromised by acute gene expression changes associated with tumor devascularization and resulting ischemia. Approximately 3,000 neoplastic and benign prostate epithelial cells were isolated using laser capture microdissection from snap-frozen prostate biopsy specimens provided by 31 patients who subsequently participated in a clinical trial of preoperative chemotherapy. To eliminate potential dye bias, we randomly alternated Cy3 and Cy5 labeling to neoplastic and benign epithelium across 31 samples. Labeled cDNA probes were hybridized in a head-to-head fashion, normal versus neoplastic from the same individual, to custom-made microarrays composed of 6,200 clones derived from the Prostate Expression Database.

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Sox9 transcriptionally regulates Wnt signaling in intestinal epithelium stem cells under hypomethylated crypts in diabetic state

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Gene regulation by Sox9 in the conjunctival epithelium

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