Project description:Gene content comparison of control C.j. strain 11168 which colonizes and causes disease in a murine model versus strain NW which colonizes but does not elicit disease symptomology in the mouse model. Keywords: DNA/DNA comparison
Project description:Multi-genome, time series transcriptome measurements across the budding yeast cell cycle 378 genome-wide microarray measurements, 18 timepoints, nine strains of S. cerevisiae and one strain of S. paradoxus Dye-swap technical replication at each strain,timepoint pair in a common reference design
Project description:Incubation of craving is a phenomenon characterized by the progressive augmentation of drug craving over a period of extended abstinence. Gene expression analyses can be leveraged to define transcriptomic changes associated with neural adaptions occurring during abstinence, informing understanding of mechanisms underlying craving and relapse. Previously, we identified differential disruptions to the nucleus accumbens transcriptome between acute and extended morphine abstinence timepoints in a rat model. Here, we expand this work to further test effects of morphine abstinence on gene expression in the ventral tegmental area (VTA), in both females and males. We additionally compare small RNA expression from plasma exosomes collected from a separate cohort of heroin-exposed males at the same acute and extended abstinence timepoints. Alterations to the VTA transcriptome diverged significantly between abstinence timepoints and sexes; specifically, VTA gene expression was altered at the acute abstinence timepoint in both sexes, but at the extended abstinence timepoint only in females, contrasting our previous findings in NAc. In heroin-abstinent males, exosomal small RNA was also altered exclusively during acute abstinence. This research expands understanding of VTA-specific regulation of abstinence and craving, in addition to identifying candidate systemic biomarkers for opioid exposure.
Project description:The data describe longitudinal profiling, pre and post treatment of a human B lymphoblastoid cell line (B-LCL). The cells were treated with Everolimus at timepoint 7 and the full untargeted proteomics and transcriptomics were profiled over a total of 24 timepoints (over 12 hours), with samples acquired once every half-hour. A validation experiment was repeated as a separate experiment using a subset of the time points.
Project description:Multi-genome, time series transcriptome measurements across the budding yeast cell cycle 378 genome-wide microarray measurements, 18 timepoints, nine strains of S. cerevisiae and one strain of S. paradoxus
Project description:We profile gene expression changes in a mutant strain lacking the D. melanogaster HP1 homolog HP1C at the third instar larval stage. Compared to the yw control strain (previously published) several hundred genes are deregulated.
Project description:Transcription analysis of M. tuberculosis H37Ra devR overexpressing strain (LIX48) versus empty vector control strain (LIX47); H37Rv devR overexpressing strain t (LIX50) versus empty vector control strain (LIX49) to study the effect of DevR overexpression