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Identification by RNA-Seq of PhoP and polymyxin B resistant regulons in Photorhabdus luminescens TT01.

ABSTRACT: A phoP mutant of the entomopathogenic bacterium P. luminescens is attenuated in virulence in insects and susceptible to antimicrobial peptides such as Polymyxin B. The first goal of this study is to compare transcriptomes of the phoP mutant and wild type strain to identify the PhoP regulon. (i) We first compared both strains grown in LB medium. (ii) As we know that low Mg conditions induce expression of PhoP-dependent genes, we also compared transcriptomes of the phoP and wild-type strain after growth in M9 low Mg and in M9 High Mg. To decipher the polymyxin B regulon, comparisons of transcriptomes of the wild type strain grown in LB medium with or without addition of polymyxin B were also performed

ORGANISM(S): Photorhabdus laumondii subsp. laumondii TTO1

PROVIDER: GSE76559 | GEO | 2017/03/10

SECONDARY ACCESSION(S): PRJNA307974

REPOSITORIES: GEO

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PhoP regulon in pathogenic Escherichia coli

Project description:PhoP is considered a regulator of virulence despite being conserved in both pathogenic and non-pathogenic Enterobacteriaceae. While Escherichia coli strains represent both non-pathogenic commensal isolates and numerous virulent pathotypes, the PhoP virulence regulator has only been studied in commensal E. coli. To better understand how conserved transcription factors contribute to virulence, we characterized PhoP in pathogenic E. coli. Loss of phoP significantly attenuated E. coli during extraintestinal infection. This was not surprising since we demonstrated that PhoP differentially regulated the transcription of >600 genes. In addition to survival at acidic pH and resistance to polymyxin B, PhoP was required for repression of motility and oxygen-independent changes in the expression of primary dehydrogenase and terminal reductase respiratory chain components. All phenotypes have in common a reliance on an energized membrane. Thus, we hypothesized that PhoP mediated these effects by regulating genes that generate a proton motive force. Indeed, bacteria lacking PhoP exhibited a hyper-polarized membrane, and dissipation of the transmembrane electrochemical gradient increased the susceptibility of the phoP mutant to acidic pH, while inhibiting respiratory generation of the proton gradient restored resistance to antimicrobial peptides independent of lipopolysaccharide modification. These findings demonstrate a connection between PhoP, virulence, and the energized state of the membrane. Comparison of gene expression between wild-type CFT073 and a CFT073 phoP deletion mutant during logarithmic phase growth in LB medium. Three biological replicates were compared from each strain.

2011-07-28 | E-GEOD-31029 | biostudies-arrayexpress

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Transcriptionnal analysis of a Photorhabdus luminescens TT01 variant, named VAR*, which is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis.

Project description:Photorhabdus luminescens lives in a mutualistic association with entomopathogenic nematodes and is pathogenic for insects. Variants of Photorhabdus frequently arise. VAR* is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis. In this study, we evaluated the role of transcriptomic modulation in determining the phenotypic variation and delayed pathogenicity of VAR* with respect to the corresponding wild-type form, TT01alpha. A P. luminescens microarray was used to determine changes in transcript profiles in the exponential and stationary phase growth phase of VAR* and TT01alpha grown in LB broth.

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M. tuberculosis phoP mutant lacking mcr7

Project description:The PhoPR two-component system is essential for virulence in Mycobacterium tuberculosis where it controls expression of approximately 2% of the genes, including those for the ESX-1 secretion apparatus, a major virulence determinant. Mutations in phoP lead to compromised production of pathogen-specific cell wall components and attenuation both ex vivo and in vivo. The PhoP regulon comprises several transcriptional regulators as well as genes for polyketide synthases and PE/PPE proteins. The most prominent site of PhoP regulation was located in the intergenic region between rv2395 and PE_PGRS41, where the mcr7 gene codes for a small non-coding RNA (ncRNA). Our prediction suggested that Mcr7 might regulate tatC at the post-transcriptional level by occlusion of the RBS and the consequent translational arrest. Consequently, we studied the secretome from exponentially grown cultures of strain H37Rv, its phoP mutant and a phoP complemented mutant by in-depth proteomics. The results are consistent with a regulatory model involving PhoP, Mcr7 and tatC mRNA since the absence of Mcr7 in the phoP mutant would result in more efficient TatC translation and therefore increased secretion.

2018-08-29 | PXD000698 | Pride

Photorhabdus laumondii subsp. laumondii TTO1

Project description:identification by RNA-Seq of PhoP and polymyxin B resistant regulons in Photorhabdus luminescens TT01.

| PRJNA307974 | ENA

Genome-wide transcriptional response of an Avian Pathogenic Escherichia coli (APEC) Pst Mutant

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2008-09-15 | GSE9178 | GEO

Generation of Escherichia coli Enhanced Acetate Utilization through Compensatory ATP Consumption via Adaptive Laboratory Evolution

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DNA adenine methyltransferase (Dam) overexpression impairs Photorhabdus luminescens motility and virulence

Project description:Dam, the most described bacterial DNA-methyltransferase, is widespread in gamma-proteobacteria. Dam DNA methylation can play a role in various genes expression and is involved in pathogenicity of several bacterial species. In the entomopathogenic bacterium Photorhabdus luminescens, a dam ortholog was identified. Overexpression of dam in P. luminescens did not impair growth ability in vitro. In contrast, compared to a control strain harboring an empty plasmid, a significant decrease in motility was observed in the dam-overexpressing strain. In addition, the dam-overexpressing P. luminescens strain showed a delayed virulence compared to that of the control strain after injection in larvae of the lepidopteran Spodoptera littoralis. These results reveal that Dam plays a major role during P. luminescens insect infection.

2017-10-04 | GSE100650 | GEO

Large chromosomal rearrangements associated with recombination between homologous prophage regions are a common feature in E. coli O157 strains and promote phenotypic plasticity in the population

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