Project description:The POLYCOMB proteins are required for maintenance of silent chromatin states mediated by H3K27 trimethylation in animals, but POLYCOMB homologues are not found in plant genomes. Using DamID-chip, we found that the Arabidopsis chromodomain-containing protein LHP1 localizes to chromatin associated with H3K27me3 genome-wide. Furthermore, the LHP1 chromodomain binds H3K27me3 with high affinity. These results suggest that LHP1 shares similar functions with POLYCOMB. Keywords: DamID-Chip
Project description:By RNA deep sequencing, around 11,000 genes were found to be differentially expressed in ebs shl lhp1 compared with wild type, and SHL and EBS, together with LHP1 and EMF1, co-regulate the expression of thousands of gene in Arabidopsis
Project description:Transcriptome changes in leaves of PcG protein mutants clf and lhp1. Fully expanded rosette leaf 6 was collected from 10 35-d-old plants grown in short day photoperiods.
Project description:RNA-seq was performed in order to identify qualitative variation of transcripts in clf-29 lhp1-4 compared to WT, including SNPS, indels, splicing fidelity
Project description:We analyzed here the genome-wide expression changes in roots of clf-29 lhp1-4 mutants in comparison to the WT reference. Plants were grown hydroponically under low nitrate provision (0.3 mM KNO3) and short-days conditions and roots were collected after 5 weeks of growth.