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Single cell transcriptome analysis of mouse carotid body glomus cells

ABSTRACT: The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNASeq method by performing next-generation sequencing on single glomus cellderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. The wealth of information provided through this study offers a valuable foundation for identifying molecules functioning in the CB.

ORGANISM(S): Mus musculus Mus musculus x Mus spretus

PROVIDER: GSE76579 | GEO | 2016/03/15

SECONDARY ACCESSION(S): PRJNA308083

REPOSITORIES: GEO

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Single cell transcriptome analysis of mouse carotid body glomus cells

Project description:The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNAÂSeq method by performing next-generation sequencing on single glomus cellÂderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. The wealth of information provided through this study offers a valuable foundation for identifying molecules functioning in the CB. 8 single CB glomus cells that were confirmed expressing tyrosine hydroxylase (Th), ubiquitin carboxyl-terminal esterase L1 (Uchl1) and potassium channel subfamily K member 3 (Kcnk3) were harvested from P4-5 wild type mice and subjected to single-cell RNA-Seq

2016-03-15 | E-GEOD-76579 | biostudies-arrayexpress

Expression data from carotid body glomus cells and neuroblasts, from young adult rats (9-11 weeks of age)

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Comparative analysis of transcriptomes from the carotid body, adrenal medulla, and superior cervical ganglion of adult mice

Project description:Acute O2 sensing is a fundamental property of cells in the peripheral chemoreceptors, e.g. glomus cells in the carotid body (CB) and chromaffin cells in the adrenal medulla (AM), and is necessary for adaptation to hypoxia. The mechanisms underlying the detection of changes in O2 tension by these cells are still poorly understood. Recently, we suggested that CB glomus cells have specific metabolic features that favour their sensitivity to hypoxia (Fernandez-Aguera et al, 2015, Cell Metab.). To investigate the metabolic profile characteristic of acute O2-sensing cells, we used adult mice to compare the transcriptomes of three organs derived from common sympathoadrenal progenitors, but exhibiting variable responsiveness to acute hypoxia: CB and AM which are O2-sensitive (CB > AM) and superior cervical ganglion (SCG), which are practically O2-insensitive. Our data support the concept that responsiveness to acute hypoxia depends on a “signature metabolic profile” in peripheral chemoreceptor cells.

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Gene expression analysis in the adult carotid body stem cell niche

Project description:The carotid body (CB) is a peripheral chemoreceptor organ that suffers a profound angiogenic and neurogenic process in response to hypoxemia, to contribute to physiological adaptation of the organism. We have previously described a subpopulation of neural crest-derived stem cells residing in the organ. These cells are responsible for hypoxia-induced neurogenesis. Little is known about the molecular mechanisms underlying this neurogenic process. We have used neuronal cell-enriched neurosphere (NS) cultures vs. undifferentiated cell-enriched NS to study molecular pathways involved in the biology of these CB different cell types.

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Expression profile of mouse carotid body and adrenal medulla [Affymetrix]

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Single cell transcriptome analysis of mouse carotid body glomus cells

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