Project description:We examined genome-wide DNA methylation with 22 human iPSC lines derived from different cell types and human ESC lines using Illumina’s Infinium HumanMethylation27 and focused on aberrant methylation sites in iPSCs for up to 42-week continuous cultivation. The iPSCs exhibited distinct epigenetic distances from ESCs at early passage. Continuous passaging of the iPSCs diminishes these differences between iPSCs and ESCs.

2011-04-14 | GSE24676 | GEO

DNA methylation landscapes of human fetal development

Project description:Here we assessed the genome-scale DNA methylation of 472,703 autosomal CpG sites in 4 tissues (muscle, amnion, pancreas and adrenal glands) on 3 time-points (9, 18 and 22 weeks) using the Illumina 450k array. We identified 52,134 CpG sites with dynamic DNA methylation during fetal development (gain or loss >20% from 9 to 22 weeks), where the period between 9 and 18 weeks was most dynamic. Loss in DNA methylation (25,579 CpGs), displayed clear tissue-specific patterns near genes enriched for a role in tissue-specific processes. Gain in DNA methylation (26,555 CpGs) occurred often near genes involved in (tissue-specific) developmental processes.

2015-09-22 | GSE56515 | GEO

Effect of epigenotoxic chemicals on DNA methylation profiles of human induced pluripotent stem cells [methylation]

Project description:We studied the effects of chemical exposure on global DNA methylation by using mCherry-methyl-CpG-binding domain (MBD)-nls human induced pluripotent stem cells (iPSCs). According to the fluorescent MBD parameters, we divided the effects of 135 chemical on global DNA methylation into 3 categories; reduction (hypomethylation), increase (hypermethylation) and little/no effect. We performed DNA methylome assay to examine the effects of 6 epigenotoxic chemicals on genome-wide DNA methylation patterns in human iPSCs. Our results indicated that hypermethylation agents increased DNA methylation and downregulated the expression of genes involved in cell cycle or development.

2021-07-23 | GSE180453 | GEO

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