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Stress-dependent dynamics of chromatin remodeling in yeast: Dual roles for SWI/SNF in heat shock stress response

ABSTRACT: Total H4 and acetyl H4 IP, time course Total H4 and acetyl H4 occupancy, Snf2 occupancy, gene expression analysis Keywords: stress response, time course

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE7665 | GEO | 2008/03/17

SECONDARY ACCESSION(S): PRJNA99731

REPOSITORIES: GEO

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Stress-dependent dynamics of chromatin remodeling in yeast: Dual roles for SWI/SNF in heat shock stress response

Project description:Total H4 and acetyl H4 IP, time course Total H4 and acetyl H4 occupancy, Snf2 occupancy, gene expression analysis Keywords: stress response, time course S cerevisiae were subjected to heat shock and stationary phase stress. The histone occupancy and H4 acetylation levels were monitored in parallel with gene expression studies, and Snf2 occupancy before and after stress.

2010-05-26 | E-GEOD-7665 | biostudies-arrayexpress

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Swi/Snf dynamics on stress responsive genes is governed by competitive bromodomain interactions.

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Composition and Function of Mutant Swi/Snf Complexes.

Project description:The 12-subunit Swi/Snf chromatin remodeling complex is conserved from yeast to humans. It functions to alter nucleosome positions by either sliding nucleosomes on DNA or evicting histones. Interestingly, 20% of all human cancers carry mutations in subunits of the Swi/Snf complex. Many of these mutations cause protein instability and loss, resulting in partial Swi/Snf complexes. Although several studies have shown that histone acetylation and activator-dependent recruitment of Swi/Snf regulate its function, it is less well understood how subunits regulate stability and function of the complex. Using functional proteomic and genomic approaches, we have assembled the network architecture of yeast Swi/Snf. In addition, we find that subunits of the Swi/Snf complex regulate occupancy of the catalytic subunit Snf2, thereby modulating gene transcription. Our findings have direct bearing on how cancer-causing mutations in orthologous subunits of human Swi/Snf may lead to aberrant regulation of gene expression by this complex.

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Composition and Function of Mutant Swi/Snf Complexes.

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Loss of Snf5 and the formation of an aberrant SWI/SNF complex

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