Transcriptomics,Genomics

Dataset Information

28

Silencing of ASCL1 in U87MG glioma cells


ABSTRACT: ASCL1 is known to act as transcriptional activator of notch signaling pathway. We have found that ASCL1 is over expressed in secondary glioblastoma. In order to delineate its functional role in gliomagensis we have used gene silencing approach to identify genes differentially regulated upon ASCL1 down regulation in U87MG glioma cell line. Overall design: U87MG cells either mock silenced using scrambled si RNA or silenced using siRNA targetted against ASCL1 were harvested at 48hrs post transfection. Gene expression was profiled after microarray for control and ASCL1 silenced cells.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array [CDF: Brainarray HGU133Plus2_Hs_ENTREZG_v15]

SUBMITTER: sainitin donakonda  

PROVIDER: GSE76652 | GEO | 2017-05-22

SECONDARY ACCESSION(S): PRJNA308293

REPOSITORIES: GEO

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Publications

System analysis identifies distinct and common functional networks governed by transcription factor ASCL1, in glioma and small cell lung cancer.

Donakonda Sainitin S   Sinha Swati S   Dighe Shrinivas Nivrutti SN   Rao Manchanahalli R Satyanarayana MRS  

Molecular bioSystems 20170701 8


ASCL1 is a basic Helix-Loop-Helix transcription factor (TF), which is involved in various cellular processes like neuronal development and signaling pathways. Transcriptome profiling has shown that ASCL1 overexpression plays an important role in the development of glioma and Small Cell Lung Carcinoma (SCLC), but distinct and common molecular mechanisms regulated by ASCL1 in these cancers are unknown. In order to understand how it drives the cellular functional network in these two tumors, we gen  ...[more]

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